食源性致病菌现场即时检测技术研究进展  

Recent Advances in Point-of-Care Diagnosis for Foodborne Pathogens

作　　者：张偲偲 刘兴泉[3] 杨媚婷 戢爽[1] 郭志军[1] 杜衍[2] ZHANG Si-Cai;LIU Xing-Quan;YANG Mei-Ting;JI Shuang;GUO Zhi-Jun;DU Yan(College of Agriculture,Yanbian University,Yanji 133000,China;State Key Laboratory of Electroanalytical Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,China;College of Agriculture and Food Science,Zhejiang Agricultural&Forestry University,Hangzhou 311300,China)

机构地区：[1]延边大学农学院,延吉133000 [2]中国科学院长春应用化学研究所电分析化学国家重点实验室,长春130022 [3]浙江农林大学农业与食品科学学院,杭州311300

出　　处：《分析化学》2021年第10期1631-1639,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.31960506,21874129);吉林省科技厅国际科技合作项目(No.20200801044GH)资助。

摘　　要：食源性致病菌如大肠杆菌、沙门氏菌、金黄色葡萄球菌等是食源性疾病和食物中毒的主要原因,通常污染水果、蔬菜、肉类和海鲜,严重威胁食品安全。食源性致病菌的快速检测对公共卫生和食品安全至关重要。然而,传统的检测方法繁琐耗时,难以满足快检快诊的需求。现场即时检测(POCT)技术作为一种新兴的现场快速检测分析技术,具有操作简单、快速、便携式和自动化等优点。POCT技术作为近年来发展迅速的食源性病原菌检测方法,为低成本、高灵敏度和高特异性检测食源性病原体提供了一种新途径。目前,POCT使用的技术主要包括光学技术和生物传感器技术,主要基于免疫学反应和生化反应。本文综述了近年来基于比色、纸基、微流控、电磁传感及市售便携式读数平台的POCT技术在食源性致病菌检测中的应用研究现状,并展望了其未来的发展前景和方向。Food safety is a global health issue.The food-borne pathogens,such as Escherichia coli,Salmonella,Staphylococcus aureus,etc.,are the main causes of food-borne diseases and food poisoning.The contamination of different foods(Like fruits,vegetables,meat and seafood)by the food-borne pathogens has threatened the food safety so long.The rapid detection of food-borne pathogens is critical to public health and food safety.However,traditional detection methods are mostly cumbersome and time-consuming,and are difficult to meet the needs of quick diagnosis and quick examination.Point-of-care testing(POCT)technology,as an emerging on-site rapid detection and analysis technology,shows many advantages such as simple operation,fast,portable and automated.POCT technology,as a food-borne pathogen detection method that has developed rapidly in recent years,provides a new way for low-cost,high-sensitivity and highspecificity detection of food-borne pathogens.At present,the technologies used in POCT are mainly divided into optical technologies and biosensor technologies,which are mainly based on immunological and biochemical reactions.This review reports the current research status of the application of POCT technology based on colorimetry,paper-based,microfluidic,electromagnetic sensing,and commercially available portable reading platforms in the detection of food-borne pathogens in recent years.

关 键 词：食品安全 食源性致病菌 现场即时检测 综述 

分 类 号：TS207.4[轻工技术与工程—食品科学]