超声辅助酶法制备草鱼鱼鳞胶原蛋白肽  

Preparation of Collagen Peptide from Grass Carp Scales by Ultrasonic-assisted Enzymatic Method

作　　者：林艳云[1] 吾惠婷 LIN Yanyun(Fuzhou University Zhicheng College,Fuzhou 350002,China)

机构地区：[1]福州大学至诚学院,福建福州350002

出　　处：《安徽农学通报》2021年第20期65-68,共4页Anhui Agricultural Science Bulletin

基　　金：福建省中青年教师教育科研项目(项目编号:JT180804)。

摘　　要：通过单因素试验分别考察了不同蛋白酶、底物浓度、加酶量、缓冲液pH、水解温度、水解时间、超声功率和超声时间对鱼鳞水解物DPPH自由基清除率的影响,并经正交试验对水解工艺进行优化。结果表明:最优制备工艺参数为:碱性蛋白酶、底物浓度10%、加酶量9000U/g、缓冲液pH 11.0、水解温度50℃、水解时间3.0h、超声功率300W、超声时间25min,在该工艺参数下制备的鱼鳞胶原蛋白肽的DPPH自由基清除率为61.4%。In this study,the effects of different protease,substrate concentration,enzyme dosage,buffer pH,hydrolysis temperature,hydrolysis time,ultrasonic power and ultrasonic time on DPPH free radical scavenging rate of fish scale hydrolysate were investigated by single factor experiment.The hydrolysis process was optimized by orthogonal experiment.The optimal extraction parameters were as follows:alkaline protease,substrate concentration 10%,enzyme dosage 9000U/g,pH value of buffer 11.0,hydrolysis temperature 50℃,hydrolysis time 3.0h,ultrasonic power 300W,ultrasonic time 25min.DPPH free radical scavenging rate of fish scale collagen peptide was 61.4%.

关 键 词：草鱼鱼鳞 胶原蛋白肽 超声波 酶解 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]