白酒醇酸酯比率对小鼠酒后乙醇代谢及行为能力的影响  

Effect of alcohols,acids and esters ratio in Baijiu on ethanol metabolism and behavioral ability of mice after drinking

作　　者：彭斌 张敬媛 何松贵 刘幼强 吴振强[1] PENG Bin;ZHANG Jingyuan;HE Songgui;LIU Youqiang;WU Zhenqiang(School of Biology and Biological Engineering,South China University of Technology,Guangzhou 510006,China;Pan Asia(Jiangmen)Institute of Biological Engineering and Health,Jiangmen 529000,China;Guangdong Jiujiang Distillery Co.,Ltd.,Foshan 528203,China)

机构地区：[1]华南理工大学生物科学与工程学院,广东广州510006 [2]江门市泛亚生物工程与健康研究院,广东江门529000 [3]广东省九江酒厂有限公司,广东佛山528203

出　　处：《中国酿造》2022年第1期103-108,共6页China Brewing

基　　金：广东省科技创新战略专项资金项目(2018 JK 35202003);广东省九江酒厂有限公司产学研合作基金(2019A00307013)。

摘　　要：控制白酒中醇、酸、酯的含量是白酒生产企业的关键技术。该研究模拟白酒中主要醇类、酸类和酯类的含量及比例配制成实验酒样,测定小鼠酒后在不同时间点的血液乙醇含量,计算小鼠的乙醇代谢能力;同时通过Morris水迷宫实验测定小鼠相应的行为能力,并测定小鼠酒后2 h时体内乙醇代谢相关酶活性以及急性酒精性肝损伤情况。结果表明,杂醇对小鼠的乙醇代谢及行为记忆能力有较大影响;过高的杂醇(尤其是异戊醇)含量,会抑制乙醇在体内代谢,主要原因是抑制了乙醇脱氢酶和乙醛脱氢酶的活性;同时降低小鼠记忆能力,显著延长潜伏逃脱时间(P<0.05)。乙酸能较好的促进乙醇代谢,减缓其他组分对乙醇脱氢酶和乙醛脱氢酶活性的抑制作用,且会刺激小鼠记忆能力,显著降低潜伏逃脱时间(P<0.05)。Controlling the ratio of alcohols,acids and esters in Baijiu(Chinese liquor)is a key technology for Baijiu production enterprises.In this study,the experimental liquor samples were configured by simulating the contents and ratios of main alcohols,acids and esters in Baijiu,and the ethanol contents in blood of mice were measured at different time points after drinking to calculate their ethanol metabolism ability.Meanwhile,the corresponding behavioral abilities of mice were measured by Morris water maze test,and the activities of enzymes related to ethanol metabolism and acute alcoholic liver injury in mice were measured at 2h after drinking.The results showed that fusel alcohols had a great effect on ethanol metabolism and behavioral memory abilities of mice.High levels of fusel alcohols(especially isoamyl alcohol)would inhibit the metabolism of ethanol in vivo,mainly because it inhibited the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase,and would reduce the memory ability of mice and significantly prolong the time of latent escape(P<0.05).Acetic acid could promote ethanol metabolism better,slow down the inhibition of other components on the activities of alcohol dehydrogenase and acetaldehyde dehydrogenase,stimulate the memory ability of mice,and significantly decrease the time of latent escape(P<0.05).

关 键 词：白酒 醇酸酯比率 酒后行为能力 乙醇代谢 小鼠模型 

分 类 号：TS261[轻工技术与工程—发酵工程]