母乳婴儿源植物乳杆菌BF_15体外抗氧化活性及缓解DSS诱导小鼠结肠氧化损伤的研究  

Studies on the in Vitro Antioxidant Activity and Alleviation of DSS-induced Oxidative Damage in Mice Colon of Lactobacillus plantarum BF_15 Derived from Breast Milk

作　　者：张娜 赵丽娜[1,3] 李晨 牛志华[1,3] 康红艳 田洪涛 罗云波 Zhang Na;Zhao Lina;Li Chen;Niu Zhihua;Kang Hongyan;Tian Hongtao;Luo Yunbo(College of Food Science and Technology,Agricultural University of Hebei,Baoding 071000,Hebei;College of Biochemistry&Environmental Engineering,Baoding University,Baoding 071000,Hebei;National Engineering Research Center for Agriculture in Northern Mountainous Areas,Baoding 071000,Hebei;New Hope Hebei Tianxiang Dairy Co.,Ltd.,Baoding 071000,Hebei;Hebei Technology Innovation Center of Probiotic Functional Dairy Product,Baoding 071000,Hebei;College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083)

机构地区：[1]河北农业大学食品科技学院,河北保定071000 [2]保定学院生物化工与环境工程学院,河北保定071000 [3]国家北方山区农业工程技术研究中心,河北保定071000 [4]河北新希望天香乳业有限公司,河北保定071000 [5]河北省益生功能性乳制品技术创新中心,河北保定071000 [6]中国农业大学食品科技与营养工程学院,北京100083

出　　处：《中国食品学报》2022年第1期67-77,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31140065);河北省重点研发计划项目(19227134D);河北省自然科学基金项目(C2017204094)。

摘　　要：以鼠李糖乳杆菌GG(LGG)为对照菌株,研究了自行分离鉴定的母乳婴儿源益生菌植物乳杆菌BF_(1)5的体外抗氧化活性、安全性与缓解DSS诱导小鼠结肠氧化损伤的症状。结果表明:试验菌株植物乳杆菌BF_(1)5能够耐受高浓度的H2O2(3.5 mmol/L)。发酵上清液中,菌株BF_(1)5对DPPH的清除能力低于对照菌株LGG(P<0.01);对·OH清除能力高于LGG(P<0.05);对·O2-清除能力高于LGG(P>0.05);抗脂质过氧化能力低于LGG(P<0.01)。菌悬液中,菌株BF_(1)5对DPPH和·O2-的清除能力高于对照菌株LGG(P>0.05);对·OH清除能力高于LGG(P<0.01);抗脂质过氧化能力低于LGG(P<0.01)。试验菌株BF_(1)5与对照菌株LGG均不产生有害代谢产物(生物胺、亚硝酸盐、吲哚),不具有溶血性。动物实验中,菌株BF_(1)5通过早期灌胃小鼠,减轻了小鼠肠黏膜损伤及炎症细胞浸润的程度,提高了超氧化物歧化酶(SOD)活性(P<0.01),降低了丙二醛(MDA)含量(P<0.01),进而有效缓解了DSS诱导小鼠结肠氧化损伤的症状,其效果与对照菌株LGG相当(P>0.05)。本研究为来自人体母乳婴儿源益生菌的开发、应用及其早期干预缓解溃疡性结肠炎(Ulcerative colitis,UC)等肠道疾病提供了科学依据,也为氧化应激作为治疗UC等肠道疾病的新靶点提供了借鉴指导。In this study,with Lactobacillus rhamnosus GG(LGG) as control strain,probiotic probiotic Lactobacillus plantarum BF_15,isolated from breast milk infant,was used as the research object to evaluate its antioxidant activity in vitro,safety and alleviation of the DSS-induced oxidative damage in the colon of mice.The results show that the test strain Lactobacillus plantarum BF_15 could tolerate high concentrations of H2 O2(3.5 mmol/L).In the fermentation supernatant,the scavenging ability of strain BF_15 on DPPH was lower than that of the control strain LGG(P<0.01);the scavenging ability of oOH was higher than LGG(P<0.05);the scavenging ability of ·O2-was higher than LGG(P>0.05);anti-lipid peroxidation ability was lower than LGG(P<0.01).In the bacterial suspension,the scavenging ability of strain BF_15 on DPPH and ·O2-was higher than that of the control strain LGG(P>0.05);the scavenging ability on ·OH was higher than LGG(P<0.01);the anti-lipid peroxidation ability was lower than LGG(P<0.01).Meanwhile,Neither the test strain BF_15 nor the control strain LGG produced harmful metabolites(bioamine,nitrite,indole),and had hemolytic properties.In animal experiments,the strain BF_15 could reduce the degree of intestinal mucosal damage and inflammatory cell infiltration,increase the activity of superoxide dismutase(SOD)(P<0.01),and reduce the level of malondialdehyde(MDA) content(P <0.01) through early intragastric administration,which effectively alleviated the symptoms of DSS-induced oxidative damage in the colon of mice;its effect was equivalent to that of the control strain LGG(P>0.05).This study provided a scientific basis for the development and application of probiotics derived from human breast milk infants and their early intervention to relieve intestinal diseases such as ulcerative colitis(UC).This study laid a solid foundation for probiotics in the prevention of intestinal diseases such as ulcerative colitis(UC),and provided a reference guide for oxidative stress as a new therapeutic target.

关 键 词：母乳婴儿源益生菌 植物乳杆菌 抗氧化活性 氧化应激 DSS诱导的溃疡性结肠炎 

分 类 号：TS201.3[轻工技术与工程—食品科学]