响应面耦合遗传算法优化桑葚花色苷的纯化工艺及抗氧化活性  

Optimization of purification process of anthocyanins from mulberry by response surface coupled genetic algorithm and its antioxidant activity

作　　者：李俊儒[1] 王静霞[1] 胡继红[1] LI Jun-ru;WANG Jing-xia;HU Ji-hong(Department of Alcohol and Food Engineering,Sichuan Vocational College of Industry and Commerce,Chengdu 611830,Sichun,China)

机构地区：[1]四川工商职业技术学院酒类与食品工程系,四川成都611830

出　　处：《粮食与油脂》2022年第2期109-115,共7页Cereals & Oils

基　　金：四川工商职业技术学院院级课题(2019NC11)。

摘　　要：以桑葚为原料,首先比较8种大孔树脂对花色苷纯度和回收率的影响,筛选出纯化花色苷的最佳树脂;然后通过单因素试验和响应面耦合遗传算法优化桑葚花色苷的纯化工艺;采用高效液相色谱(high performance liquid chromatography,HPLC)分析纯化前后花色苷提取物组分,并测定纯化前后花色苷提取物对ABTS^(+)·和·OH的清除能力。结果表明:AB-8大孔树脂为纯化桑葚花色苷的最佳大孔树脂。桑葚花色苷最优的纯化工艺参数为上样液质量浓度2.4 mg/m L、径长比1:23、洗脱液乙醇体积分数70%、洗脱流速3.3 mL/min。在此条件下,桑葚花色苷纯度为(21.46±0.05)%,花色苷在纯化前后组分未发生改变。花色苷粗提物和纯化物对ABTS^(+)·和·OH清除率的IC_(50)分别为(5.83±0.05)、(7.08±0.07)和(5.35±0.04)、(7.72±0.05)mg/mL。Using mulberry as raw material,the effects of 8 macroporous resins on anthocyanin purity and recovery were compared,and the best resin for anthocyanin purification was selected.Then the purification process of mulberry anthocyanin was optimized by single factor test and response surface coupled genetic algorithm.High performance liquid chromatography(HPLC)was used to analyze the components of anthocyanin extracts before and after purification,and the scavenging ability of anthocyanin extracts before and after purification on ABTS^(+)·and·OH was determined.The results showed that AB-8 macroporous resin was the best macroporous resin for the purification of mulberry anthocyanin.The optimal purification parameters were as follows:the mass concentration of loading solution 2.4 mg/mL,diameter to length ratio 1:23 and the volume fraction of ethanol in elution 70%,and the elution flow rate 3.3 mL/min.Under these conditions,the purity of mulberry anthocyanin was(21.46±0.05)%,and the components of anthocyanin did not change before and after purification.The IC_(50)of anthocyanin crude extract and purified product for ABTS^(+)·and·OH clearance were(5.83±0.05),(7.08±0.07)and(5.35±0.04),(7.72±0.05)mg/mL,respectively.

关 键 词：桑葚 花色苷 纯化工艺 抗氧化活性 

分 类 号：TS201.1[轻工技术与工程—食品科学]