酵母菌与乳酸菌发酵马乳产ACE抑制肽  

Optimized Co-fermentation of Horse Milk by Yeast and Lactic Acid Bacteria to Produce Angiotensin-converting Enzyme-Inhibiting Peptides

作　　者：唐蓉 王康 郭元晟[2] 刘彦敏 孙建萍[2] 朱建军[2] 伊日布斯[1] TANG Rong;WANG Kang;GUO Yuansheng;LIU Yanmin;SUN Jianping;ZHU Jianjun;CHAGAN Irbis(Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming 650500,China;Institute of Biological Engineering,Xilingol Vocational College,Xilinhot 026000,China)

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500 [2]锡林郭勒职业学院生物工程研究院,内蒙古锡林浩特026000

出　　处：《食品科学》2022年第6期236-245,共10页Food Science

基　　金：昆明理工大学科研项目(KKK0201826056)。

摘　　要：通过使用从传统酸马乳中分离纯化和筛选出的乳酸菌与酵母菌共发酵马乳,获得具有血管紧张素转换酶(angiotensin-converting enzyme,ACE)抑制活性的生物活性肽。经过发酵性能和生长性能测试后筛选到发酵马乳ACE抑制率为38.67%的乳酸乳球菌(Lactococcus lactis)L8和ACE抑制率为51.33%解脂耶氏酵母(Yarrowia lipolytica)Y7进行组合发酵,单因素试验和响应面试验优化发酵条件后发酵马乳的ACE抑制率达到80.67%。与单菌发酵相比,ACE抑制率显著提高。对共发酵产物进行分离纯化,得到了ACE抑制率为86%的活性组分,发酵马乳是分离制备ACE抑制肽的潜在来源。This study aims to obtain biologically active peptides with angiotensin-converting enzyme(ACE)-inhibiting activity by co-fermenting horse milk with lactic acid bacteria and yeasts isolated from traditional Koumiss.Lactococcus lactis L8 and Yarrowia lipolytica Y7,fermented milk with which inhibited ACE activity by 38.67%and 51.33%,were used for mixed-strain fermentation of horse milk.Horse milk fermented under optimum conditions determined using one-fact-at-a-time method and response surface methodology inhibited ACE activity by as high as 80.67%.The ACE-inhibiting activity significantly increased compared with that obtained with single-strain fermentation.An active fraction with ACE-inhibiting rate of 86%was separated from the co-fermented product.In conclusion,fermented horse milk is a potential source of ACE-inhibiting peptides.

关 键 词：共发酵 马乳 高血压 血管紧张素转换酶 小分子肽 

分 类 号：TS201.3[轻工技术与工程—食品科学]