甘薯渣多糖的提取工艺优化、结构鉴定及其功能活性研究  

Study on Extraction Process Optimization,Structure Identification and Functional Activity of Polysaccharide from Sweet Potato Residue

作　　者：段旭 冉军舰[1] 孙俊良[1] 梁新红[1] 刘剑桥 王慧敏 王济良 DUAN Xu;RAN Junjian;SUN Junliang;LIANG Xinhong;LIU Jianqiao;WANG Huimin;WANG Jiliang(Henan Institute of Science and Technology of Food Science and Technology,Xinxiang 453003,China;Administration for Market Regulation of Hongqi District,Xinxiang 453003,China)

机构地区：[1]河南科技学院食品学院,河南新乡453003 [2]新乡市红旗区质量监管局,河南新乡453003

出　　处：《食品工业科技》2022年第8期228-237,共10页Science and Technology of Food Industry

基　　金：河南省高校重点科研项目(21A550009)。

摘　　要：本研究采用超声波辅助热水浸提法提取甘薯渣粗多糖,经单因素实验和响应面优化提取工艺参数,并通过酶法脱蛋白、H_(2)O_(2)法脱色和Superose 1210/300 GL凝胶柱对多糖进行分离纯化,得到甘薯渣多糖。采用紫外光谱法、红外光谱法和高效液相色谱法对甘薯渣多糖进行结构鉴定,在此基础上进一步对甘薯渣多糖进行抗氧化活性测定以及体外降血糖能力测定。结果表明,甘薯渣多糖的最佳提取工艺条件为提取温度70℃、超声功率264 W、提取时间56 min、料液比1:17 g/mL,在此条件下多糖得率为5.053%。经红外鉴定表明该多糖为α-吡喃葡萄糖,高效液相测定表明其由9.46%甘露糖、4.28%鼠李糖、11.72%葡萄糖醛酸、62.37%葡萄糖和10.58%木糖组成。测定其体外抗氧化活性发现,甘薯渣多糖清除DPPH·、·OH、超氧阴离子的IC_(50)值分别为3.089、4.879、5.832 mg/mL,对其体外降血糖测定发现,甘薯渣多糖抑制α-淀粉酶以及α-葡萄糖苷酶的IC_(50)值分别为7.674、18.961 mg/mL。综上,甘薯渣多糖具有良好的抗氧化、降血糖等功能活性。上述研究结果为甘薯渣多糖的纯化、功能活性分析及其综合利用提供了数据参考。In this study,ultrasonic assisted hot water extraction was used to extract crude polysaccharides from sweet potato residue.Single factor experiments and response surface optimization were used to extract the technological parameters.The polysaccharides from sweet potato residue were obtained by enzymatic deproteinization,H_(2)O_(2) decolorization and Superose 1210/300 GL gel column.The structure of sweet potato residue polysaccharide was identified by ultraviolet spectroscopy,infrared spectroscopy and HPLC.On this basis,the antioxidant activity and hypoglycemic ability of sweet potato residue polysaccharide were determined in vitro.The experimental results showed that the optimum extraction conditions of polysaccharide from sweet potato residue were extraction temperature 70℃,ultrasonic power 264 W,extraction time56 min and solid-liquid ratio 1:17 g/mL.under these conditions,the yield of polysaccharide was 5.053%.Infrared identification showed that the polysaccharide was α-glucopyranose was determined by HPLC.It was composed of 9.46% mannose,4.28% rhamnose,11.72% glucuronic acid,62.37% glucose and 10.58% xylose.The IC_(50) values of DPPH·,·OH and superoxide anion scavenged by sweet potato residue polysaccharide were 3.089,4.879,5.832 mg/mL respectively,the IC_(50) values of α-amylase and α-glucosidase were 7.674 and 18.961 mg/mL,respectively.In summary,the sweet potato residue polysaccharide had good antioxidant and hypoglycemic activities.The above results would provide data reference for the purification,functional activity analysis and comprehensive utilization of sweet potato residue polysaccharide.

关 键 词：甘薯渣多糖 分离纯化 结构鉴定 抗氧化活性 降血糖活性 

分 类 号：TS209[轻工技术与工程—食品科学]