红曲废渣发酵制备枯草芽孢杆菌微生态制剂  

Production of Bacillus subtilis Microbioecologics by Fermentation of Hongqu Residue

作　　者：张晨[1,2,3] 王扬 杨新 潘丹丹 胡文林 时祥柱 陈炳钿 罗春连[7] 倪莉 ZHANG Chen;WANG Yang;YANG Xin;PAN Dandan;HU Wenlin;SHI Xiangzhu;CHEN Bingtian;LUO Chunlian;NI Li(Institute of Food Science and Technology,Fuzhou University,Fuzhou 350108,China;Fujian Food Biotechnology Innovation Engineering Technology Research Center,Fuzhou 350108,China;Food Nutrition and Health Research Center,Fuzhou University,Jinjiang 362251,China;Guangdong Tianyi Biological Technology Co.,Ltd.,Zhanjiang 524300,China;Fujian Xinminke Biological Technology Development Co.,Ltd.,Fuzhou 350008,China;Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350012,China;Fujian Industrial Products Production License Review Technology Center,Fuzhou 350013,China)

机构地区：[1]福州大学食品科学技术研究所,福建福州350108 [2]福建省食品生物技术创新工程技术研究中心,福建福州350108 [3]福州大学食品营养与健康研究中心,福建晋江362251 [4]广东省天益生物科技有限公司,广东湛江524300 [5]福建省新闽科生物科技开发有限公司,福建福州350008 [6]福建省农业科学院畜牧兽医研究所,福建福州350012 [7]福建省工业产品生产许可证审查技术中心,福建福州350013

出　　处：《食品工业科技》2022年第10期140-148,共9页Science and Technology of Food Industry

基　　金：福建省科技厅高校产学合作项目(2019N5002);福建省科技计划星火项目(2019S0002)。

摘　　要：本研究通过单因素和响应面分析优化红曲废渣制备枯草芽孢杆菌微生态制剂的条件,对发酵过程中可溶性糖和可溶性蛋白质的含量进行追踪,以分析菌体增长的限制因素,并对该微生态制剂的储藏稳定性和体外抗氧化性进行评估。结果表明,当发酵条件为:红曲废渣浓度100 g/L,枯草芽孢杆菌接种量为10%(5 lg CFU/mL),摇床转速为220 r/min时,在37℃条件下发酵48 h可获得枯草芽孢杆菌最高产量为(9.3±0.3)lg CFU/mL。红曲废渣中可溶性葡萄糖的含量是限制枯草芽孢杆菌生长的主要因素。当可溶性糖含量较低时,枯草芽孢杆菌的生长基本停止。根据加速保存实验可计算出微生态制剂在20℃下保存3个月后的活菌数为8.7 lg CFU/mL,高于应用标准。微生态制剂具有较强的抗氧化性,其DPPH自由基清除能力为0.25 mg/mL(IC_(50)),羟自由基的清除能力为0.69 mg/mL(IC_(50))。本研究证明红曲废渣可作为原料制备枯草芽孢杆菌微生态制剂,为微生物发酵废渣的可持续性绿色加工提供了实践依据。In this study, single factor and response surface analysis were used to optimize the conditions for the production of Bacillus subtilis using Hongqu residue, the content of soluble sugar and soluble protein components during the fermentation were determined to analyze the limiting factors of bacterial growth, and the storage stability and the in vitro antioxidant activity of the microbioecologics were determined. The results showed that the highest yield of Bacillus subtilis in microbioecologics was(9.3±0.3) lg CFU/mL that was produced at 37 ℃ for 48 h with the conditions of 100 g/L Hongqu residue, and shaking speed of 220 r/min with 10% inoculation volume of 5 lg CFU/mL Bacillus subtilis. The content of soluble glucose in Hongqu residue was the main factor limiting the growth of Bacillus subtilis. When soluble sugar contents were low, the growth of Bacillus subtilis basically stopped. According to the accelerated preservation experiment, the viable count of probiotics stored at 20 ℃ for three months was 8.7 lg CFU/mL, which was higher than the criteria of microbioecologics application. The microbioecologics had strong antioxidant activity that scavenging capacity of DPPH free radical and hydroxyl free radical were 0.25 mg/mL(IC_(50)) and 0.69 mg/mL(IC_(50)), respectively. This study proved that Hongqu residue could be used as a raw material to prepare Bacillus subtilis probiotics, which provided a practical basis for the sustainable green processing of microbial fermentation residue.

关 键 词：红曲废渣 枯草芽孢杆菌 微生态制剂 储藏稳定性 抗氧化活性 

分 类 号：TS201.1[轻工技术与工程—食品科学]