壁材对真空低温喷雾干燥制备乳双歧杆菌Probio-M8微胶囊的影响  

The Effects of Wall Materials on the Preparation of Bifidobacterium lactis Probio-M8 Microcapsules by Vacuum Low Temperature Spray Drying

作　　者：郭帅 卢家君 孟和毕力格[1] Guo Shuai;Lu Jiajun;Menghe Bilige(Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Inner Mongolia Agricultural University,Hohhot 010018)

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室,呼和浩特010018

出　　处：《中国食品学报》2022年第5期219-227,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家现代农业产业技术体系;内蒙古自治区科技成果转化专项(2019CG027)。

摘　　要：目的:研究不同壁材对真空低温喷雾干燥制备乳双歧杆菌Probio-M8微胶囊的影响。方法:对不同壁材微胶囊粉末的溶解度、再水合时间、粉末流动性以及粉末水分和水分活度的物理性质进行测定,使用荧光显微镜观测干燥前、后菌体细胞膜完整性的变化,扫描电镜观察微胶囊粉的形态结构,差示扫描量热仪测定微胶囊粉的玻璃化转变温度,并测定了不同壁材菌体的DPPH自由基清除能力和总抗氧化能力。结果:复合壁材的Probio-M8微胶囊粉末表现出较高的存活率,为73.8%,且具有较低的水分含量(4.85%)和水分活度(0.17),粉末流动性也优于其它3种微胶囊粉末,然而,由于复合壁材组分复杂,复合壁材的微胶囊粉具有较低的溶解度和较长的再水合时间。此外,复合壁材保护的Probio-M8细胞膜的完整性也高于其它3种壁材保护的Probio-M8,有效地保护了菌体细胞膜的完整性,并具有较高的玻璃化转变温度和较强的抗氧化能力,有利于Probio-M8微胶囊粉的贮藏稳定性。结论:通过比较不同壁材对Probio-M8真空低温喷雾干燥后菌体活性的影响,发现不同的壁材对菌体有不同的保护效果,其中复合壁材可更有效保护菌体的活性。研究结果对乳双歧杆菌Probio-M8微胶囊的工业化生产有一定的参考意义。Objective:The effects of different wall materials on the preparation of Bifidobacterium lactis Probio-M8 microcapsules by vacuum low-temperature spray drying were studied in this study.Method:The solubility,rehydration time,powder fluidity,and the physical properties of the powder moisture and water activity of the microcapsule powder with different wall materials were measured by physical methods,and the changes in the integrity of the bacterial cell membrane before and after drying were observed using a fluorescence microscope.The morphology and structure of the microcapsule powder were observed by scanning electron microscope and the glass transition temperature of the microcapsule powder was measured with a differential scanning calorimeter.The DPPH free radical scavenging ability and total antioxidant capacity of different wall materials to protect the bacteria were measured.Results:The Probio-M8 microcapsule powder of composite wall material showed a higher survival rate of 73.8%,and had a lower water content(4.85%)and water activity(0.17),and the powder had excellent fluidity.But due to the complex composition of the composite wall material,the microcapsule powder of the composite wall material had lower solubility and longer rehydration time than the three kinds of microcapsule powders.In addition,the integrity of the Probio-M8 cell membrane protected by the composite wall material was also higher than that of the Probio-M8 protected by the other three wall materials,which effectively protects the integrity of the bacterial cell membrane.Furthermore,the Probio-M8 microcapsule powder of composite wall material had a higher glass transition temperature and a higher glass transition temperature and strong antioxidant capacity which is beneficial to the storage stability of Probio-M8 microcapsule powder.Conclusion:This experiment compared the effects of different wall materials on the bacterial activity after spray drying of Probio-M8,and found that different wall materials have different protective effects o

关 键 词：壁材 真空低温喷雾干燥 微胶囊 

分 类 号：TS201.3[轻工技术与工程—食品科学]