枯草芽胞杆菌发酵制备南极磷虾肽及其体外抗氧化活性研究  

Preparation of Antarctic krill peptides by fermentation of Bacillus subtilis and its in vitro antioxidant activity

作　　者：杨柳 郑汉丰[1] 郭全友[1] 杨絮[1] 周国燕 郑尧[1] YANG Liu;ZHENG Hanfeng;GUO Quanyou;YANG Xu;ZHOU Guoyan;ZHENG Yao(East Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;School of Health Sciences and Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090 [2]上海理工大学健康科学与工程学院,上海200093

出　　处：《食品与发酵工业》2022年第14期84-92,共9页Food and Fermentation Industries

基　　金：国家重点研发计划“蓝色粮仓科技创新”专项(2020YFD0901204);中国水产科学研究院基本科研业务费资助项目(2020TD68)。

摘　　要：采用枯草芽胞杆菌发酵脱脂南极磷虾粉,制备南极磷虾蛋白肽,通过单因素和正交试验对发酵条件进行优化,并对所得粗多肽进行体外抗氧化活性测定。结果表明,在保障多肽得率的基础上,高水解度的最佳发酵条件为发酵时间3 d、料液比3∶100(g∶mL)、初始接种量1.5×10^(8) CFU/mL,高蛋白回收率的最佳发酵条件为发酵时间3 d、料液比1∶100(g∶mL)、初始接种量1.5×10^(8) CFU/mL。结果表明,2种发酵条件下所得的南极磷虾粗多肽均具有良好的抗氧化活性,其中高水解度发酵组的总抗氧化能力更强,高蛋白回收率发酵组的自由基清除率更强。综上,利用枯草芽胞杆菌发酵制备具有良好抗氧化性的南极磷虾蛋白肽是可行的,为南极磷虾的精深加工和综合利用提供参考。Antarctic krill protein peptides were prepared by fermentation of the defatted Antarctic krill powder with Bacillus subtilis.The fermentation conditions were optimized by single-factor and orthogonal tests,and the in vitro antioxidant activity of the crude peptides was determined.The results showed that the optimal fermentation conditions for high hydrolysis were 3 d,3∶100(g∶mL)stock-to-liquid ratio and 1.5×10^(8) CFU/mL initial inoculum,and the optimal fermentation conditions for high protein recovery were 3 d,1∶100(g∶mL)stock-to-liquid ratio and 1.5×10^(8) CFU/mL initial inoculum.The total antioxidant capacity was stronger in the high hydrolysis fermentation group,and the free radical scavenging rate was stronger in the high protein recovery fermentation group.It showed that the preparation of Antarctic krill protein peptides with good antioxidant properties by fermentation with Bacillus subtilis is feasible and provides a reference for the deep processing and comprehensive utilization of Antarctic krill.

关 键 词：南极磷虾粉 枯草芽胞杆菌 发酵 蛋白肽 体外抗氧化活性 

分 类 号：TS254.4[轻工技术与工程—水产品加工及贮藏工程]