水酶法提取红花籽油的工艺优化及其抗氧化性研究  

Study on process optimization and antioxidant activity of aqueous enzymatic extraction of safflower seed oil

作　　者：范康俊 吴士筠[1] 艾廷阳 刘虹[1] 严兴初[2] 覃瑞[1] FAN Kang-jun;WU Shi-jun;AI Ting-yang;LIU Hong;YAN Xing-chu;QIN Rui(College of Life Science,South-Central Minzu University,Hubei Provincial Key Laboratory for Protection and Application of Special Plants Germplasm in Wuling Area of China,Wuhan 430070,Hubei,China;Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,Hubei,China)

机构地区：[1]中南民族大学生命科学学院,武陵山区特色资源植物种质保护与利用湖北省重点试验室,湖北武汉430070 [2]中国农业科学院油料作物研究所,湖北武汉430062

出　　处：《粮食与油脂》2022年第8期61-65,94,共6页Cereals & Oils

基　　金：国家自然科学基金(32072123);湖北省科技创新条件共享平台项目(2021DFE021)。

摘　　要：以红花籽仁为原料,采用水酶法提取红花籽油,并研究其抗氧化活性。在单因素试验基础上,通过响应面法分析,优化提取工艺参数。结果表明:在料液比1∶4(g/mL)、酶用量5%(以加水量计)、复合酶的质量比2∶1∶3、酶解时间4 h的条件下,红花籽油提取率可达77.60%±0.05%。红花籽油脂肪酸组成以不饱和脂肪酸为主,其中亚油酸、油酸、亚麻酸的相对含量分别为76.51%、11.03%、0.13%;红花籽油对DPPH·的IC_(50)为4.133 mg/mL,具有较好的抗氧化能力。With safflower seed as raw material,safflower seed oil was extracted by aqueous enzymatic method,and its antioxidant activity was studied.On the basis of single factor test,the experimental parameters were optimized by response surface method.The results showed that under the conditions of solid-liquid ratio 1∶4(g/mL),composite enzyme mass ratio 2∶1∶3,enzyme dosage 5%(calculated by wa-ter mass)and enzymatic hydrolysis time 4 h,the yield of safflower seed oil was reached 77.60%±0.05%.The fatty acid composition of safflower seed oil was mainly unsaturated fatty acid,and the relative content of linoleic acid,oleic acid and linolenic acid reached 76.51%,11.03%and 0.13%,respectively.The IC_(50) of safflower seed oil to DPPH·was 4.133 mg/mL,indicating that safflower seed oil had good antioxidant capacity.

关 键 词：水酶法 红花籽油 响应面法 脂肪酸成分 抗氧化活性 

分 类 号：TS225.19[轻工技术与工程—粮食、油脂及植物蛋白工程]