自溶对双孢菇多糖体外抗氧化和抗肿瘤活性的影响  

Autolysis Alters the in Vitro Antioxidant and Antitumor Activities of Agaricus bisporus Polysaccharides

作　　者：孙晓燕 郭慧 余君彤 张广文[1] 彭喜春[1] SUN Xiaoyan;GUO Hui;YU Juntong;ZHANG Guangwen;PENG Xichun(College of Science and Engineering,Jinan University,Guangzhou 510000,China)

机构地区：[1]暨南大学理工学院,广东广州510000

出　　处：《食品科学》2022年第17期74-83,共10页Food Science

基　　金：广东省重点领域研发计划项目(2020B020226008)。

摘　　要：本研究旨在探究自溶对双孢菇多糖(Agaricus bisporus polysaccharides,ABP)的分子质量以及体外抗氧化和抗肿瘤活性的影响。通过对在不同温度和时间下发生自溶的双孢菇进行多糖提取,分离纯化具有强抗氧化和抗肿瘤活性的ABP组分,测定分子质量并分析单糖组成。结果表明,与不自溶组相比,ABP由于自溶降解,出现了更多的中分子质量多糖。自溶作用增强了低分质子量多糖的体外抗氧化和抗肿瘤活性。具有最佳抗氧化活性和体外抗肿瘤活性的多糖分子质量为619.72 Da,主要的单糖组成为葡萄糖、半乳糖和鼠李糖。This study aimed to investigate the effect of Agaricus bisporus autolysis on the molecular mass,in vitro antioxidant and antitumor activities of Agaricus bisporus polysaccharides(ABP).After different periods of Agaricus bisporus autolysis at different temperatures,ABP were extracted and fractionated to obtain fractions with strong antioxidant and antitumor activities,and the molecular mass and monosaccharide composition of the bioactive fractions were determined.The results showed that ABP were degraded to medium-molecular-mass polysaccharides due to Agaricus bisporus autolysis.Agaricus bisporus autolysis enhanced the antioxidant and antitumor activities of low-molecular-mass polysaccharides.The molecular mass of the ABP fraction with the best antioxidant and antitumor activities was 619.72 Da and monosaccharide composition analysis showed that it was mainly composed of glucose,galactose,and rhamnose.

关 键 词：双孢菇 多糖 自溶 抗氧化 抗肿瘤 

分 类 号：TS201.1[轻工技术与工程—食品科学]