富硒酵母发酵工艺的优化  

Optimization of fermentation technology of selenium-riched yeast

作　　者：涂青 杨双全[3] 章之柱 陈秋慧 卢红梅[1,2] 徐海粟 仇治艳 陈莉 TU Qing;YANG Shuangquan;ZHANG Zhizhu;CHEN Qiuhui;LU Hongmei;XU Haisu;QIU Zhiyan;CHEN Li(Guizhou Key Lab of Fermentation Engineering and Biological Pharmacy,Guizhou University,Guiyang 550025,China;School of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China;School of Chemistry and Chemical Engineering,Guizhou University,Guiyang 550025,China)

机构地区：[1]贵州大学贵州省发酵工程与生物制药重点实验室,贵州贵阳550025 [2]贵州大学酿酒与食品工程学院,贵州贵阳550025 [3]贵州大学化学与化工学院,贵州贵阳550025

出　　处：《中国酿造》2022年第10期140-145,共6页China Brewing

基　　金：贵州省科技支撑计划(黔科合支撑[2019]2317)。

摘　　要：以富硒酿酒酵母(Saccharomyces cerevisiae)SY2为发酵菌种,利用5 L发酵罐培养富硒酵母。以富硒酵母生长及硒转化率为评价指标,优化其发酵工艺条件,比较溶氧反馈补料与拟指数补料两种方式对富硒酵母生长、硒转化率等的影响。结果表明,富硒酵母最适发酵条件为:初始pH 5.0,发酵温度30℃,接种量8%,硒添加量30 mg/L,发酵时间24 h。在此优化条件下,富硒酵母生物量为9.8 g/L,硒转化率为77.5%。在拟指数补料方式下,培养周期为34 h,富硒酵母得率为0.252 g/g,硒含量为1920.5μg/g,硒的转化率为82.7%;在溶氧反馈补料方式下,培养周期为46 h,富硒酵母得率为0.317 g/g,硒含量为1759.5μg/g,硒转化率为90.1%。结果显示,拟指数补料培养周期短,生产强度、酵母硒含量较高,是适宜补料方式。Using Saccharomyces cerevisiae SY2 as fermentation strain,the selenium-enriched yeast was fermented in a 5 L fermenter.Using the growth and selenium conversion rate of selenium-enriched yeast as evaluation indexes,the fermentation conditions were optimized,and the effects of dissolved oxygen feedback feeding and quasi-exponential feeding on the growth and selenium conversion rate of selenium-enriched yeast were compared.The results showed that the optimal fermentation conditions of selenium-enriched yeast were initial pH 5.0,fermentation temperature 30℃,inoculum 8%,selenium addition 30 mg/L and time 24 h.Under the optimized conditions,the biomass of selenium-enriched yeast was 9.8 g/L,and the selenium conversion rate was 77.5%.Under the quasi-exponential feeding method,the fermentation time was 34 h,the yield of selenium-enriched yeast was 0.252 g/g,the content of selenium was 1920.5μg/g,and the selenium conversion rate was 82.7%.Under the dissolved oxygen feedback feeding method,the fermentation time was 46 h,the yield of selenium-enriched yeast was 0.317 g/g,the content of selenium was 1759.5μg/g,and the selenium conversion rate was 90.1%.The results shown that the quasi-exponential feeding with short culture cycle,high production intensity and high yeast selenium content was the optimal feeding method.

关 键 词：富硒酵母 发酵工艺 优化 补料方式 

分 类 号：TS261.1[轻工技术与工程—发酵工程]