柠檬皮多酚成分分析及其对胰岛素抵抗HepG2细胞糖代谢的影响  

Analysis of Polyphenols from Lemon Peel and Its Effect on Glucose Metabolism in Insulin-resistant HepG2 Cells

作　　者：王瑞雪 张筠[2] 崔艳伟 付红岩[2] 房一明[1] 张彦军[1] 初众[1] WANG Ruixue;ZHANG Yun;CUI Yanwei;FU Hongyan;FANG Yiming;ZHANG Yanjun;CHU Zhong(Chinese Academy of Tropical Agricultural Sciences,Spice and Beverage Research Institute,Wanning 571533,China;College of Food Engineering,East University of Heilongjiang College,Harbin 150066,China)

机构地区：[1]中国热带农业科学院香料饮料研究所,海南万宁571533 [2]黑龙江东方学院食品工程学院,黑龙江哈尔滨150066

出　　处：《食品工业科技》2022年第23期310-317,共8页Science and Technology of Food Industry

基　　金：农业农村部香辛饮料作物遗传资源利用重点实验室、海南省香料饮料作物工程技术研究中心开放课题资助(2019xys003);黑龙江东方学院科研创新团队建设项目(HDFKYTD202104)。

摘　　要：目的:探究柠檬皮多酚(Limon Peel Polyphenols,LPP)的组成成分,并研究其对胰岛素抵抗(Insulin Resistance,IR)的HepG2细胞糖代谢的影响。方法:采用高效液相色谱串联四级杆飞行时间质谱法(HPLCQTOF-MS)分析LPP组成,利用HepG2细胞建立胰岛素抵抗模型,用LPP作用于IR-HepG2细胞,通过测定细胞葡萄糖消耗量初步探究LPP对糖代谢的影响,再通过测定糖原含量和己糖激酶(Hexokinase,HK)、丙酮酸激酶(Pyruvate Kinase,PK)、磷酸烯醇式丙酮酸羧基酶(Phosphoenol Pyruvate Carboxykinase,PEPCK)、葡萄糖六磷酸酶(Glucose-6-Phosphatase,G6Pase)的活性,探究LPP调节细胞糖代谢的作用途径。结果:经过HPLCQTOF-MS分析出12种物质,主要为黄酮及其苷类成分;在糖代谢研究方面,与模型组相比浓度为0.1~2 mg/mL柠檬皮多酚可显著提高细胞葡萄糖消耗量(P<0.05),且当浓度为0.5 mg/mL时其提高IR-HepG2细胞糖原含量和HK、PK活性,降低PEPCK和G6Pase活性的能力最接近于二甲双胍阳性对照。结论:柠檬皮多酚可以缓解IR-HepG2细胞的胰岛素抵抗状态,并能通过促进糖原合成、提高糖酵解关键酶活性、降低糖异生酶活力的方式调节糖代谢水平,为后续的体内研究提供了数据支持,并为未来开发功能性产品提供了理论依据。Objective:To explore the composition of limon peel polyphenols(LPP)and its influence on glucose metabolism in insulin resistance(IR)HepG2 cells.Methods:The composition of LPP was analyzed by HPLC-QTOF-MS.HepG2 cells were used to establish insulin resistance model,IR-HepG2 cells were treated with LPP,and the effect of LPP on glucose metabolism was preliminarily investigated by measuring glucose consumption.By determining glycogen content and hexokinase(HK),pyruvate kinase(PK),phosphoenol pyruvate carboxykinase(PEPCK)and glucose-6-phosphatase(G6Pase)activities to explore the pathway of LPP regulating glucose metabolism in cells.Results:Twelve compounds were identified by HPLC-QTOF-MS,mainly flavonoids and their glycosides.In the study of glucose metabolism,compared with the model group,the concentration of 0.1~2 mg/mL lemon peel polyphenols significantly increased glucose consumption(P<0.05),and the ability to increase the glycogen content and HK and PK activities and decrease the PEPCK and G6Pase activity of IR-HepG2 cells was closest to metformin-positive control when the concentration of lemon peel polyphenols was 0.5 mg/mL.Conclusion:Lemon peel polyphenols can reduce IR-HepG2 cell state of insulin resistance,and can promote glycogen synthesis,improve the glycolytic key enzyme activity,reduce sugar dysplasia regulate sugar metabolism enzyme activity way,it provides data support for the subsequent research in the body,and provides the theoretical basis for the future development of functional products.

关 键 词：柠檬皮多酚 成分分析 降糖活性 HEPG2 细胞 胰岛素抵抗 

分 类 号：TQ283[化学工程—有机化工]