低GI杂粮可可冲调粉辅助降血糖作用  

Auxiliary Hypoglycemic Effect of Low-GI Multigrain Cocoa Powder

作　　者：那治国[1,2] 余爽 贺书珍[3] 初众[3] NA Zhiguo;YU Shuang;HE Shuzhen;CHU Zhong(College of Food Engineering,Harbin University of Commerce,Harbin 150028,China;College of Food Engineering,East University of Heilongjiang,Harbin 150066,China;Chinese Academy of Tropical Agricultural Sciences,Spice and Beverage Research Institute,Wanning 571533,China)

机构地区：[1]哈尔滨商业大学食品工程学院,黑龙江哈尔滨150028 [2]黑龙江东方学院食品工程学院,黑龙江哈尔滨150066 [3]中国热带农业科学院香料饮料研究所,海南万宁571533

出　　处：《食品工业科技》2023年第1期28-37,共10页Science and Technology of Food Industry

基　　金：农业农村部香辛饮料作物遗传资源利用重点实验室、海南省热带香料饮料作物工程技术研究中心开放课题资助(2019xys002);黑龙江省重点研发计划指导类项目(GZ20210166);黑龙江东方学院科研项目(HDFKY200101)。

摘　　要：目的:研究低GI杂粮可可冲调粉(Multigrain Cocoa Powder,C-MGP)对糖尿病小鼠血糖的影响。方法:52只雄性SPF级小鼠随机分为6组,包括正常对照组、模型组、二甲双胍组、冲调粉低、中、高剂量组(5%、10%、30%C-MGP)。正常对照组小鼠喂养基础饲料,模型组和二甲双胍组小鼠喂养高糖高脂饲料,冲调粉低剂量组(5%C-MGP)、中剂量组(10%C-MGP)、高剂量组(30%C-MGP)小鼠分别喂养含5%(质量分数)、10%(质量分数)、30%(质量分数)C-MGP的高糖高脂饲料。高脂饲料喂养四周后,小鼠腹腔注射30 mg/kg链脲佐菌素(STZ)建立Ⅱ型糖尿病小鼠模型。造模成功后,继续喂养4周,并测定小鼠基础指标和糖脂代谢等相关指标的变化。结果:经过高脂饲料联合STZ诱导,与模型组相比,10%C-MGP和30%C-MGP摄食量、饮水量均极显著降低(P<0.01),体重极显著升高(P<0.01)。与模型组相比,30%C-MGP显著降低了STZ诱导糖尿病小鼠的肝脏、肾脏指数,降低了空腹血糖水平(FBG)、糖化血红蛋白(GHb)、空腹胰岛素水平(FINS)及胰岛素抵抗指数(HOMA-IR),提高了葡萄糖耐量(GT),改善了血清中甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)和游离脂肪酸(FFA)水平,极显著促进了肝糖原(HG)合成(P<0.01),改善了肝脏脂肪堆积,极显著提高了血清胰高血糖素样肽(GLP-1)含量(P<0.01),极显著降低了血清脂多糖(LPS)水平(P<0.01)。结论:30%C-MGP具有调节STZ诱导糖尿病小鼠的糖脂代谢,减轻糖尿病症状的作用,具有进一步开发利用的价值。Objective:To study the effect of low GI multigrain cocoa powder(C-MGP)on blood glucose in diabetic mice.Methods:Fifty-two male SPF mice were randomly divided into 6 groups,including normal control group,model group,metformin group,flushing powder low,medium and high-dose groups(5%,10%,30%C-MGP).Mice in normal control group were fed basal diet,mice in model group and metformin group were fed high-glucose and high-fat diets.Mice in low dose group(5%C-MGP),medium dose group(10%C-MGP)and high dose group(30%C-MGP)were fed high-glucose and high-fat diets containing 5%(mass fraction),10%(mass fraction)and 30%(mass fraction)low GI mixed grain cocoa,respectively.After four weeks of high-fat diet,typeⅡdiabetic mouse model was established by intraperitoneal injection of streptozotocin(STZ)(30 mg/kg).After successful modeling,feeding was continued for 4 weeks,basic indexes and glucose and lipid metabolism indexes were measured during the experiment in mice.Results:After high-fat diet combined with STZ induction,compared with the model group,10%C-MGP and 30%C-MGP had significantly lower food intake and water intake(P<0.01),and significantly higher body weight(P<0.01).Compared with the model group,30%C-MGP significantly decreased liver and kidney indexes,fasting blood glucose(FBG),glycosylated hemoglobin(GHb),fasting insulin(FINS)and insulin resistance index(HOMA-IR),and improved glucose tolerance(GT)in STZ-induced diabetic mice.Total glyceride(TG),total cholesterol(TC),low density lipid-cholesterol(LDL-C),high density lipid-cholesterol(HDL-C)and free fatty acids(FFA)levels were improved,hepatic glycogen(HG)synthesis was significantly promoted(P<0.01),and liver fat accumulation was improved.Significantly increased the content of serum glucagon like peptide-1(GLP-1)(P<0.01),significantly decreased the level of serum lipopolysaccharide(LPS)(P<0.01).Conclusion:The 30%C-MGP had the effects of regulating glucose and lipid metabolism,and reduces diabetes symptoms on SZT induced diabetic mice,which is valuable for further developme

关 键 词：杂粮 可可 降血糖 Ⅱ型糖尿病 冲调粉 

分 类 号：R587.1[医药卫生—内分泌]