不同等级高温大曲微生物群落和代谢物的特征分析  

Microbial communities and metabolites characteristics of high temperature Daqu with different grades

作　　者：丁芳 张原頔 蒋英丽 陈波 陈小雪 韩北忠[1] DING Fang;ZHANG Yuandi;JIANG Yingli;CHEN Bo;CHEN Xiaoxue;HAN Beizhong(Beijing Laboratory for Food Quality and Safety,College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083,China;Sichuan Langjiu Co.,Ltd.,Luzhou 510500,China;Department of Nutrition and Health,China Agricultural University,Beijing 100083,China)

机构地区：[1]中国农业大学食品科学与营养工程学院食品质量与安全北京实验室,北京100083 [2]四川郎酒股份有限公司,四川泸州510500 [3]中国农业大学营养与健康系,北京100083

出　　处：《中国酿造》2022年第11期24-30,共7页China Brewing

基　　金：中国酒业协会委托科研项目(202105410610989)。

摘　　要：该研究采用高通量测序(HTS)、气相色谱-离子迁移谱(GC-IMS)和氢谱核磁共振(1H NMR)比较了不同等级高温大曲微生物群落和代谢物的差异。HTS结果表明,不同等级高温大曲基于属水平的优势菌群有一定差异,一级大曲中优势菌属为慢生芽孢杆菌属(Lentibacillus)、克罗彭斯特菌属(Kroppenstedtia)、葡萄球菌属(Staphylococcus)和曲霉属(Aspergillus)等,优级大曲中优势菌属为芽孢杆菌属(Bacillus)、克罗彭斯特菌属和踝节菌属(Rasamsonia)等,特级大曲中优势菌属为芽孢杆菌属、克罗彭斯特菌属和曲霉属等。GC-IMS结果表明,不同等级高温大曲挥发性代谢物组成及含量的差异主要体现在醇类、醛酮类和酯类等物质。1H NMR检测结果表明,不同等级高温大曲中非挥发性代谢物组成及含量的差异主要体现在氨基酸等物质,且特级大曲非挥发性代谢物的种类更为丰富、含量更高。The differences of microbiotas and metabolites in high-temperature Daqu with different grades were compared using high-throughput sequencing(HTS), gas chromatography-ion mobility spectrometry(GC-IMS) and1H nuclear magnetic resonance(1H NMR). The HTS results showed that the dominant community of high-temperature Daqu with different grades had obvious differences based on genus level. The dominant genera were Lentibacillus, Kroppenstedtia, Staphylococcus and Aspergillus in the first-grade Daqu, Bacillus, Kroppenstedtia and Rasamsonia in premium-grade Daqu, and Bacillus, Kroppenstedtia and Aspergillus in super premium-grade Daqu. The GC-IMS results showed that the composition and contents of volatile metabolites were different in high-temperature Daqu with different grades, which were reflected in the content of alcohols, aldehydes, ketones and esters, etc. The1H NMR detection results showed that the differences in the composition and contents of non-volatile metabolites in high-temperature Daqu with different grades were mainly reflected in the content of amino acids, etc., and the species and contents of non-volatile metabolites of super premium-grade Daqu were more abundant and higher.

关 键 词：高温大曲 感官评价 微生物群落 代谢物 

分 类 号：TS262.3[轻工技术与工程—发酵工程]