不同环境条件对隆德假单胞菌生物被膜形成能力的影响  

Impact of Environmental Conditions on the Biofilm Formation of Pseudomonas lundensis

作　　者：刘永吉[1,2] 刘根梅 刘国凌 康林芝[1] LIU Yongji;LIU Genmei;LIU Guoling;KANG Linzhi(Henry Fok School of Food Science and Engineering,Shaoguan University,Shaoguan 512005,China;Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region,Shaoguan 512005,China)

机构地区：[1]韶关学院英东食品科学与工程学院,广东韶关512005 [2]广东省粤北食药资源利用与保护重点实验室,广东韶关512005

出　　处：《食品工业科技》2023年第4期122-128,共7页Science and Technology of Food Industry

基　　金：广东省自然科学基金项目(2018A030310041);广东省普通高校特色创新类项目(2018KTSCX206)。

摘　　要：为研究环境条件对食品腐败菌隆德假单胞菌(Pseudomonas lundensis,PL)生物被膜形成能力的影响,采用微孔板结晶紫法测定不同的营养条件、接种浓度、pH、NaCl和Mg^(2+)浓度条件下其生物被膜量,用激光共聚焦扫描显微镜观测其在不锈钢材料上的黏附和结构特征。结果表明,PL生物被膜的形成量在营养胁迫下降低,稀释TSB培养基50倍造成的营养胁迫使其生物被膜量从1.75±0.35降低至0.24±0.17。PL接种量从1.3×10^(7) CFU/mL降至2.5×10^(4) CFU/mL时,其生物被膜的形成量无显著差异(P>0.05),在pH为8.0时PL形成生物被膜量最多,1.25%以上的葡萄糖、4%以上的NaCl和0.5%的Mg^(2+)能够显著(P<0.05)抑制PL生物被膜的形成。激光共聚焦显微镜观测结果表明该菌在一定浓度下具有在不锈钢表面形成典型生物被膜的能力。PL生物被膜形成能力受营养条件、葡萄糖浓度、pH、NaCl浓度、Mg^(2+)等环境因子的影响。In order to investigate the influence of environmental conditions on biofilm formation ability of food spoilage strain Pseudomonas lundensis(PL),the biofilm amount was measured by microplate crystal violet method under different nutritional conditions,inoculation concentration,pH,NaCl and Mg^(2+)concentration conditions.The adhesion and structural characteristics of PL on stainless steel surface were investigated by laser scanning confocal microscope.The results showed that nutritional stress formed by dilute tryptone soy broth reduced the amount of PL biofilm formation,and the nutrient stress caused by 50-fold dilution of TSB medium reduced the amount of biofilm from 1.75±0.35 to 0.24±0.17.There was no significant difference in biofilm formation when PL inoculation amount decreased from 1.3×10^(7) CFU/mL to 2.5×10^(4) CFU/mL(P>0.05).The highest biofilm production was observed at pH 8.0.The biofilm formation was significantly inhibited respectively when the addition of glucose exceeded 1.25%,NaCl exceeded 4%and Mg^(2+)exceeded 0.5%.The observation results of laser scanning confocal microscopy showed that the PL had the ability to form typical biofilm on stainless steel surface at a certain concentration.The ability of PL biofilm formation was influenced by environmental factors such as nutrient stress,glucose concentration,pH,NaCl and Mg^(2+)concentration.

关 键 词：隆德假单胞菌 生物被膜 环境条件 腐败菌 

分 类 号：TS201.3[轻工技术与工程—食品科学]