Exosomes derived from human umbilical cord mesenchymal stem cells alleviate Parkinson’s disease and neuronal damage through inhibition of microglia  

作　　者：Zhong-Xia Zhang Yong-Jie Zhou Ping Gu Wei Zhao Hong-Xu Chen Ruo-Yu Wu Lu-Yang Zhou Qing-Zhuo Cui Shao-Kang Sun Lin-Qi Zhang Ke Zhang Hong-Jun Xu Xi-Qing Chai Sheng-Jun An 

机构地区：[1]Department of Neurology,the First Affiliated Hospital of Hebei Medical University,Shijiazhuang,Hebei Province,China [2]Research Center,Hebei University of Chinese Medicine,Shijiazhuang,Hebei Province,China

出　　处：《Neural Regeneration Research》2023年第10期2291-2300,共10页中国神经再生研究（英文版）

基　　金：supported by the Natural Science Foundation of Hebei Province,Nos.18967728D (to XQC),H2021423063 (to HXC);Youth Top Talent Project of Colleges and Universities in Hebei Province,No.BJ2021033 (to HXC)。

摘　　要：Microglia-mediated inflammatory responses have been shown to play a crucial role in Parkinson’s disease. In addition, exosomes derived from mesenchymal stem cells have shown anti-inflammatory effects in the treatment of a variety of diseases. However, whether they can protect neurons in Parkinson’s disease by inhibiting microglia-mediated inflammatory responses is not yet known. In this study, exosomes were isolated from human umbilical cord mesenchymal stem cells and injected into a 6-hydroxydopamine-induced rat model of Parkinson’s disease. We found that the exosomes injected through the tail vein and lateral ventricle were absorbed by dopaminergic neurons and microglia on the affected side of the brain, where they repaired nigral-striatal dopamine system damage and inhibited microglial activation. Furthermore, in an in vitro cell model, pretreating lipopolysaccharide-stimulated BV2 cells with exosomes reduced interleukin-1β and interleukin-18 secretion, prevented the adoption of pyroptosis-associated morphology by BV2 cells, and increased the survival rate of SH-SY5Y cells. Potential targets for treatment with human umbilical cord mesenchymal stem cells and exosomes were further identified by high-throughput microRNA sequencing and protein spectrum sequencing. Our findings suggest that human umbilical cord mesenchymal stem cells and exosomes are a potential treatment for Parkinson’s disease, and that their neuroprotective effects may be mediated by inhibition of excessive microglial proliferation.

关 键 词：6-HYDROXYDOPAMINE dopamine neurons EXOSOMES inflammation mesenchymal stem cells MICROGLIA Parkinson’s disease PYROPTOSIS 

分 类 号：R742.5[医药卫生—神经病学与精神病学]