超声辅助纤维素酶法提取辣木籽多酚的工艺优化及其体外活性  

Process Optimization of the Extraction of Moringa oleifera Seed Polyphenols by Ultrasound-Assisted Cellulase and Its in Vitro Activity

作　　者：胡霞 王顺风 白玉英 解静 田洋[1,2,3] HU Xia;WANG Shunfeng;BAI Yuying;XIE Jing;TIAN Yang(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China;National Rresearch Center for Moringa oleifera Lam.Processing Technology,Kunming 650201,China;Engineering Center for the Development and Utilization of Food and Medicine Homologous Resoueces,Kunming 650201,China)

机构地区：[1]云南农业大学食品科学技术学院,云南昆明650201 [2]国家辣木加工技术研发专业中心,云南昆明650201 [3]教育部食药同源开发与利用工程中心,云南昆明650201

出　　处：《食品工业科技》2023年第7期207-214,共8页Science and Technology of Food Industry

基　　金：云南省重大科技专项计划项目(202002AA100005,202102AE090027-2);国家木薯产业技术体系(CARS-11-YNTY);云南省科技厅基础研究专项(202201AT070262)。

摘　　要：本实验以辣木籽为原料,研究纤维素酶添加量、乙醇体积分数、纤维素酶解时间、超声时间对辣木籽多酚提取量的影响,并采用响应面法优化超声辅助纤维素酶法提取辣木籽多酚工艺。此外,研究辣木籽多酚的体外抗氧化和降糖降脂活性。结果表明,超声辅助纤维素酶法提取辣木籽多酚最佳工艺为:酶添加量0.30%、乙醇体积分数53.00%、酶解时间31.00 min、超声时间33.00 min,在此条件下,所得辣木籽多酚的提取量为6.90 mg/g,与预测值无显著性差异。辣木籽多酚提取物具有较好的抗氧化活性,对ABTS自由基和DPPH自由基的清除能力的IC_(50)分别为0.76和0.61 mg/mL。同时,辣木籽多酚提取物具有较好的胰脂肪酶和α-淀粉酶抑制活性,其对胰脂肪酶和α-淀粉酶抑制率的IC_(50)分别为1.35和6.55 mg/mL。该研究为辣木籽多酚提取物的提取和应用提供理论依据。In this study,the effects of cellulase addition,ethanol volume fraction,cellulase digestion time and ultrasound time on the extraction rate of Moringa oleifera seeds polyphenols were investigated.At the same time,the extraction process of Moringa oleifera seeds polyphenols by ultrasound-assisted cellulase method was optimized using response surface methodology.In addition,the in vitro antioxidant and hypoglycemic and hypolipidemic activities of Moringa oleifera seeds polyphenols were also investigated.The results showed that the optimal process for the extraction of Moringa oleifera seed polyphenols by ultrasound-assisted cellulase was 0.30% enzyme addition,53.00% volume fraction of ethanol,31.00 min enzymatic digestion time,and 31.00 min ultrasound time.Under this process condition,the amount of Moringa oleifera seed polyphenols extracted obtained was 6.90 mg/g,which was not significantly different from the predicted value.In addition,the study found that Moringa oleifera seed polyphenols had good antioxidant activity,and its IC_(50)for scavenging ABTS radicals and DPPH radicals were 0.76 and 0.61 mg/mL,respectively.Meanwhile,Moringa oleifera seed polyphenols had some pancreatic lipase and α-amylase inhibitory activities,and theirs IC_(50)for pancreatic lipase and α-amylase inhibition rate were 1.35 and 6.55 mg/mL,respectively.This study provides a theoretical basis for the extraction and application of Moringa oleifera seed polyphenols.

关 键 词：辣木籽 多酚 纤维素酶 超声 抗氧化 胰脂肪酶 Α-淀粉酶 

分 类 号：TS201.2[轻工技术与工程—食品科学]