麦芽协同复合蛋白酶催化玉米蛋白水解物制备及抗氧化活性研究  

Study on preparation and antioxidant activity of corn gluten meal hydrolysate catalyzed by malt and protamex

作　　者：周丹丹 刘晓兰[1,2,3] 邓永平 郑喜群[1,2,3] ZHOU Dan-dan;LIU Xiao-lan;DENG Yong-ping;ZHENG Xi-qun(College of Food and Biological Engineering,Qiqihar University,Qiqihar 161006,Heilongjiang,China;Key Laboratory of Corn Deep Processing Theory and Technology,Qiqihar 161006,Heilongjiang,China;College of Food Science,Heilongjiang Bayi Agricultural University,Daqing 163319,Heilongjiang,China)

机构地区：[1]齐齐哈尔大学食品与生物工程学院,黑龙江齐齐哈尔161006 [2]黑龙江省玉米深加工理论与技术重点实验室,黑龙江齐齐哈尔161006 [3]黑龙江八一农垦大学食品学院,黑龙江大庆163319

出　　处：《粮食与油脂》2023年第3期21-26,共6页Cereals & Oils

基　　金：国家重点研发计划“十三五”子课题(2017YFD0400200);中央支持地方高校改革发展资金高水平人才项目(2020GSP08)。

摘　　要：研究了麦芽协同复合蛋白酶水解玉米蛋白粉的条件,并分析了水解物的抗氧化活性。结果表明:最佳条件为先用麦芽水解,在底物质量浓度150 g/L、麦芽添加量15 g/100g、pH 5.5、温度50℃条件下水解3 h;然后用复合蛋白酶水解,在加酶量1.3 g/100 g、pH 8.0、温度60℃条件下水解2.5 h。在此条件下,蛋白质回收率为50.18%±0.24%,相比于不加麦芽,蛋白质回收率提高了11.16%。水解物蛋白质量浓度为1 mg/mL时,亚铁离子螯合率、DPPH·清除率、·OH清除率和·O_(2)^(-)清除率分别为83.90%±0.38%、51.19%±1.07%、79.30%±0.63%和35.68%±0.51%,相比于不加麦芽,亚铁离子螯合率降低0.85%,DPPH·清除率、·OH清除率和·O_(2)^(-)清除率分别提高了8.13%、24.41%和64.73%。The conditions of hydrolyzing corn gluten meal with malt and protamex were studied, and the antioxidant activity of the hydrolysate was analyzed. The results showed that the optimum conditions were as follows: first, hydrolyzing corn gluten meal with malt for 3 h under the conditions of substrate mass concentration 150 g/L, malt addition 15 g/100 g, pH 5.5, and temperature 50 ℃, then hydrolyzing with protamex for 2.5 h under the conditions of enzyme addition 1.3 g/100 g, pH 8.0 and temperature 60 ℃. Under these conditions, the protein recovery was 50.18 %±0.24 %, which was 11.16 % higher than that without malt. When the mass concentration of hydrolysate protein was 1 mg/mL, the ferrous ion chelation rate, DPPH · clearance rate, · OH clearance rate and ·O_(2)^(-)clearance rate were 83.90 %±0.38 %, 51.19 %±1.07 %, 79.30 %±0.63 % and 35.68 %±0.51 %, respectively. Compared with without malt, the ferrous ion chelation rate decreased by 0.85 %, and the DPPH· clearance rate, ·OH clearance rate and · O-2clearance rate increased by 8.13 %, 24.41 % and 64.73 % respectively.

关 键 词：玉米蛋白粉 麦芽 复合蛋白酶 水解 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]