源于皱纹盘鲍裙边胶原蛋白ACE抑制肽的制备  

Preparation of Angiotensin I-Converting Enzyme Inhibitory Peptides from Haliotis discus hannai Mantle Collagen

作　　者：刘维维 肖盼盼 陈玉磊 张凌晶[1] 翁凌[1] 刘光明[1] 曹敏杰[1] LIU Weiwei;XIAO Panpan;CHEN Yulei;ZHANG Lingjing;WENG Ling;LIU Guangming;CAO Minjie(College of Marine Food and Biological Engineering,Jimei University,Xiamen 361021,China)

机构地区：[1]集美大学海洋食品与生物工程学院,福建厦门361021

出　　处：《食品科学》2023年第6期158-164,共7页Food Science

基　　金：“十三五”国家重点研发计划重点专项(2018YFD0901004);国家自然科学基金面上项目(31772049)。

摘　　要：为了提高皱纹盘鲍(Haliotis discus hannai)裙边的利用率,以其为原料纯化胶原蛋白,并通过胰蛋白酶和胃蛋白酶共酶解制备血管紧张素转换酶(angiotensin I-converting enzyme,ACE)抑制肽。酶解液经超滤、Superdex^(TM) peptide 10/300 GL凝胶柱和高效液相色谱分离纯化,获得了3条来源于皱纹盘鲍胶原蛋白的肽段SGEVGQ、QRGPAGAQGPQ和GPPGPAGAR。其中结合能力最强的肽为GPPGPAGAR,对ACE的IC_(50)值为177.1μmol/L,分子对接结果显示其主要作用于ACE的S_(1)活性口袋,抑制模式与赖诺普利类似,并且经模拟胃肠液消化后仍能发挥较强的ACE抑制作用。本研究通过酶解皱纹盘鲍裙边胶原蛋白制备ACE抑制肽,为鲍鱼裙边的精深加工和ACE抑制肽的开发提供了参考。In order to improve the utilization of Haliotis discus hannai mantle,collagen purified from it was hydrolyzed by trypsin and pepsin to prepare angiotensin I-converting enzyme(ACE)inhibitory peptides.The hydrolysate was purified by sequential ultrafiltration,Superdex^(TM) peptide 10/300 GL gel filtration chromatography and high performance liquid chromatography(HPLC).Three ACE inhibitory peptides,SGEVGQ,QRGPAGAQGPQ and GPPGPAGAR,were obtained.Among them,GPPGPAGAR had the highest ACE inhibitory activity with a half maximal inhibitory concentration(IC_(50))of 177.1μmol/L.Molecular docking results showed that GPPGPAGAR mainly acted on the S_(1) active pocket of ACE and the inhibitory mode was similar to that of lisinopril.GPPGPAGAR still showed high ACE inhibitory activity after simulated gastrointestinal digestion.This study will provide a reference for deep processing of abalone mantle and the development of ACE inhibitory peptides.

关 键 词：鲍鱼裙边 胶原蛋白 血管紧张素转换酶抑制肽 分子对接 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]