桃金娘多酚的响应面优化提取及抗氧化活性研究  

Optimization of Extraction and Antioxidant Activity of Polyphenols from Rhodomyrtus tomentosa by Response Surface Methodology

作　　者：徐艳[1] 邱元凯[1] 何欢 罗文敏 方广平 骆玮诗 XU Yan;QIU Yuankai;HE Huan;LUO Wenmin;FANG Guangping;LUO Weishi(Heyuan Polytechnic,Heyuan 517000;Heyuan Jilongxiang Biotechnology Co.,Ltd.,Heyuan 517000)

机构地区：[1]河源职业技术学院,河源517000 [2]河源市吉龙翔生物科技有限公司,河源517000

出　　处：《食品工业》2023年第3期81-85,共5页The Food Industry

基　　金：广东省教育厅科技项目(2017GKTSCX071);广东省自然科学基金面上项目(2021A1515010803);校级指定科研项目(2017KJZ01);广东省科技厅科技计划项目(2016A020211005)。

摘　　要：采用响应面法对桃金娘多酚提取条件进行优化,研究乙醇体积分数、提取温度、料液比、提取时间等因素对桃金娘多酚提取效果的影响,并对桃金娘多酚进行体外抗氧化活性试验。结果表明,响应面优化最优提取工艺为乙醇体积分数45%、提取温度76℃、料液比1︰95 (g/mL),此工艺下可实现32.58±0.24 mg GAE/g的最高多酚提取量。此外,试验发现桃金娘多酚具备较强抗氧化功能,其清除DPPH自由基、ABTS^(+)·自由基的IC_(50)(半抑制浓度)分别是4.47μg/mL和10.93μg/mL。The response surface methodology was used to optimize the condition of polyphenols extraction from Rhodomyrtus tomentosa.The experiment explored the effects of ethanol concentration,extraction temperature,solid-to-liquid ratio and extraction time on the extraction rate of polyphenols.The results showed that the optimal condition for extraction that provided the maximum yield of polyphenols of 32.58±0.24 mg GAE/g berries was determined as follows:45%ethanol as the extraction solvent,a solid-to-liquid ratio of 1:95(g/mL),an extraction temperature of 76℃.The test of antioxidant activity showed that the polyphenols from Rhodomyrtus tomentosa had better antioxidant activity,and half inhibition concentrations(IC_(50))of ABTS^(+)and DPPH were 10.93μg/mL and 4.47μg/mL,respectively.

关 键 词：桃金娘 响应面分析 多酚 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]