玉米淀粉糖化反应影响因素研究  

Study on the Influencing Factors of Corn Starch Saccharification Reaction

作　　者：雷英杰 于孝民 任元元 邹德君 孟资宽 邹育 李宇航 LEI Ying-jie;YU Xiao-ming;REN Yuan-yuan;ZOU De-jun;MENG Zi-kuan;ZOU Yu;LI Yu-hang(Sichuan Food Fermentation Industry Research and Design Institute Co.,Ltd.,Chengdu,Sichuan 611130,China;Cofco Biochemical(Chengdu)Co.,LTD,Chengdu,Sichuan 611130,China)

机构地区：[1]四川省食品发酵工业研究设计院有限公司,四川成都611130 [2]中粮生化(成都)有限公司,四川成都611130

出　　处：《粮油食品科技》2023年第3期48-54,共7页Science and Technology of Cereals,Oils and Foods

基　　金：四川粮油加工关键技术及产品开发(2020YFN0148)。

摘　　要：目前在玉米淀粉糖工业生产过程中,糖化反应阶段存在初始淀粉乳浓度低、反应时间长、酶解效果不佳、最终产物得率较低、杂糖成分较多等问题。针对以上现象,以40%浓度淀粉乳作为原料对糖化反应阶段的温度、pH值、加酶量等参数进行研究,以正交设计得出最佳糖化条件。同时分析糖化底物葡萄糖当量(DE值)对糖化反应及糖化产物组成的影响。结果表明,影响糖化反应的因素主次为pH>加酶量>底物DE值,糖化酶最佳反应的条件为温度60℃,pH值为4.5,加酶量200 U/g,底物DE值为15。该条件下糖化液黏度适中,更有利于酶解反应的进行,最终产物DE值可达95%以上,葡萄糖含量(DX值)最高可达96.86%,且杂糖含量仅为1.30%。研究结论能够为玉米淀粉糖工业化生产提升产物得率、降低成本消耗提供理论参考。At present,in the industrial production process of corn starch sugar,there are some problems in the saccharification reaction stage,such as low initial starch milk concentration,long reaction time,poor enzymatic hydrolysis effect,low yield of final product,and more miscellaneous sugar components.Aiming at the above phenomena,the optimal saccharification conditions were obtained by orthogonal design with the parameters of the saccharification reaction stage,such as temperature,pH value,and the amount of enzyme added by using starch milk with 40%concentration as raw material.At the same time,the effect of DE value of glycosylated substrate on the glycosylation reaction and the composition of glycosylated products was analyzed.The results showed that the main factors affecting the glycosylation reaction were pH>enzyme amount>substrate DE value.The optimum conditions for the reaction of glycosylase were temperature 60℃,pH 4.5,enzyme dosage 200 U/g and substrate DE 15.Under this condition,the viscosity of the saccharification solution was moderate,which was more conducive to the enzymolysis reaction.The DE value of the final product could reach 95%and the glucose content(DX value)could reach 96.86%.Meanwhile,the content of other components was only 1.30%.The conclusion of this study can provide theoretical reference for the industrial production of corn starch sugar to improve product yield and reduce cost.

关 键 词：淀粉糖 糖化工艺 酶解 黏度 产物得率 

分 类 号：TS245.5[轻工技术与工程—制糖工程] S-3[轻工技术与工程—食品科学与工程]