基于非靶向代谢组学技术筛选植物乳植杆菌P9抑黄曲霉物质  

Analysis of the Inhibitory Effect of Lactiplantibacillus plantarum P9 on Aspergillus flavus Using Non-targeted Metabonomics

作　　者：陈佳敏 张文羿[1] 孟和毕力格[1] Chen Jiamin;Zhang Wenyi;Menghe Bilige(Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Key Laboratory of Dairy Products Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Agricultural University,Hohhot 010018)

机构地区：[1]内蒙古农业大学,乳品生物技术与工程教育部重点实验室,农业农村部奶制品加工重点实验室,呼和浩特010018

出　　处：《中国食品学报》2023年第5期87-95,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金地区科学基金项目(31660450)。

摘　　要：为解析共培养下植物乳植杆菌P9代谢产生的抑黄曲霉物质,本研究将植物乳植杆菌P9与黄曲霉进行共培养处理,分析植物乳植杆菌P9单菌及共培养中活菌数的差异,依据活菌数差异,分别在共培养9 h和13 h取样,进行非靶向代谢组学分析,并对取样点的差异代谢物及其通路富集情况进行研究。结果显示:共培养下植物乳植杆菌P9可以显著抑制黄曲霉,共培养产生的差异代谢物主要集中在能量代谢途径,包括:糖酵解/糖异生途径,柠檬酸循环,丙酮酸代谢途径,氨基酸代谢途径,叶酸生物合成途径及有机酸代谢途径。与已报道的植物乳植杆菌产生的抑菌物质进行比对,鉴定到的差异代谢物中可能对黄曲霉起拮抗作用的物质主要为2-氨基-3-羟基丁酸、十六烷酸、2,3-二羟基苯甲酸等有机酸,以及杆菌肽等氨基酸类物质,本研究为深入探寻植物乳植杆菌抑黄曲霉物质提供参考。To analyze the inhibitory effect of Aspergillus flavus on the metabolism of Lactiplantibacillus plantarum P9 under co cultivation,this study co cultured L.plantarum P9 with A.flavus.The differences in viable counts between L.plantarum P9 monoculture and co-culture were analyzed,and based on the differences in viable counts,samples were taken at 9 h and 13 h of co-culture for non-targeted metabolomic analysis,and the enrichment of differential metabolites and their pathways at the sampling sites were investigated.The results showed that L.plantarum P9 could significantly inhibit A.flavus,and the differential metabolites produced by co-culture were mainly in the energy metabolism pathway,including:glycolysis/gluconeogenesis pathway,citric acid cycle,pyruvate metabolism pathway,amino acid metabolism pathway,folate biosynthesis pathway and organic acid metabolism pathway.In comparison with the reported inhibition substances produced by L.plantarum,the identified differential metabolites that may play an antagonistic role against A.flavus are mainly organic acids such as 2-amino-3-hydroxybutyric acid,hexadecanoic acid,2,3-dihydroxybenzoic acid,and amino acid substances such as bacillus peptides,and this study provides a reference for further research on the inhibition substances of A.flavus by L.plantarum.

关 键 词：植物乳植杆菌P9 黄曲霉 液体共培养 非靶向代谢组学 代谢差异 

分 类 号：TS201.3[轻工技术与工程—食品科学]