魔芋粗粉制备低聚糖工艺优化及产物生理活性分析  

Optimization of Oligosaccharide Preparation Process and Physiological Activity Analysis of Konjac Crude Powder

作　　者：张薇 孙楠 张钰瑶 伍小丫 赵育 张冰倩 张宝善[1] ZHANG Wei;SUN Nan;ZHANG Yuyao;WU Xiaoya;ZHAO Yu;ZHANG Bingqian;ZHANG Baoshan(College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi’an 710119,China)

机构地区：[1]陕西师范大学食品工程与营养科学学院,陕西西安710119

出　　处：《食品工业科技》2023年第18期147-155,共9页Science and Technology of Food Industry

基　　金：益生菌发酵果品功能饮料关键技术研究与示范(2023-YBNY-195);陕西特色果品干制及精深加工关键技术研究与示范(2023-ZDLNY-42)。

摘　　要：本文利用地衣芽孢杆菌(Bacillus licheniformis)水解魔芋粗粉制备低聚糖,通过单因素实验确定魔芋粗粉浓度、菌株接种量、水解温度、水解时间4个因素对魔芋粗粉水解率的影响,在此基础上利用响应面优化水解条件。利用粘度计、薄层层析(TLC)分析水解产物特性,以·OH、·O_(2)^(-)和DPPH·清除能力为指标评价魔芋粗粉低聚糖抗氧化活性,以体外发酵试验探究魔芋粗粉低聚糖益生元活性。结果表明,水解最佳工艺参数为魔芋粗粉浓度20 g/L、接菌量1×10^(7)CFU/g、水解温度43.6℃、水解时间10.2 h,此时,魔芋粗粉水解率为39.06%±0.12%,魔芋葡甘低聚糖(Konjac glucomannan oligosacchrrides,KGOS)产率为66.72%。随着水解时间延长,溶液粘度由2430.33 mPa·s下降到18.06 mPa·s。魔芋粗粉浓度10 mg/mL时,·OH、·O_(2)^(-)和DPPH·清除率分别为61.47%、34.60%和81.43%。KGOS、葡萄糖和魔芋粗粉对植物乳杆菌、短乳杆菌和干酪乳杆菌体外增殖作用OD600分别为0.775、1.371、0.236,0.791、1.339、0.224和0.916、1.336、0.243。该结果为魔芋粗粉低聚糖开发利用提供了依据。In this paper,konjac powde were hydrolyzed by Bacillus licheniformis to prepare oligosaccharides.The effects of concentration of konjac powder,inoculum amount of strain,bacteriolysis temperature and hydrolysis time on hydrolysis rate of konjac powder were determined by single factor tests.The antioxidant activity of konjac powder oligosaccharides was evaluated with the scavenging ability of·OH,·O_(2)^(-)·and DPPH·as indexes.The prebiotic activity of konjac powder oligosaccharides was investigated by in vitro fermentation test.The results showed that the optimal hydrolysis process parameters was as follows:concentration of konjac powder 20 g/L,inoculation capacity 1×10^(7)CFU/g,hydrolysis temperature 43.6℃,hydrolysis time 10.2 h.Under the optimal conditions,the hydrolysis rate of konjac powder was 39.06%±0.12%,andtheyieldofKGOSwas66.72%.Withtheprolongationofhydrolysistime,theviscosityofsolutiondecreasedfrom 2430.33 mPa·s to 18.06 mPa·s.When the concentration of konjac powder was 10 mg/mL,the clearance rates of·OH,-·O2 and DPPH·were 61.47%,34.60%and 81.43%,respectively.The OD600 of KGOS,glucose and konjac powder on the proliferation of Lactobacillus plantarum,Lactobacillus brevity and Lactobacillus casei in vitro were 0.775,1.371,0.236,0.791,1.339,0.224 and 0.916,1.336,0.243,respectively.The results provide a theoretical basis for the further processing and utilization of oligosaccharides in konjac powder.

关 键 词：魔芋葡甘低聚糖 抗氧化活性 益生元 生理活性 薄层层析 

分 类 号：TS201.2[轻工技术与工程—食品科学]