稳定同位素内标-高效液相色谱串联质谱法测定植物油中香兰素、甲基香兰素、乙基香兰素和乙基麦芽酚  

Determination of vanillin,methyl vanillin,ethyl vanillin and ethyl maltol in vegetable oil by high performance liquid chromatography-tandem mass spectrometry with stable isotope internal standard

作　　者：刘川 林浩 姚静 刘蓉 肖全伟 戴琴 LIU Chuan;LIN Hao;YAO Jing;LIU Rong;XIAO Quanwei;DAI Qin(Chengdu Institute of Food Inspection,Chengdu 611130)

机构地区：[1]成都市食品检验研究院,成都611130

出　　处：《中国食品添加剂》2023年第9期271-278,共8页China Food Additives

摘　　要：建立同位素稀释-高效液相色谱串联质谱法测定植物油中香兰素、甲基香兰素、乙基香兰素和乙基麦芽酚含量的方法。样品经乙腈水提取,氯化钠盐析,C18色谱柱分离后采用多反应监测模式(MRM)检测,内标法定量。香兰素、甲基香兰素、乙基香兰素和乙基麦芽酚在2.0~100.0 ng/mL浓度范围内线性关系良好,平均加标回收率介于97.0%~107.7%之间,相对标准偏差介于2.46%~10.1%(n=6)之间。该方法使用内标法消除基质效应、提高数据准确性,适用于植物油中香兰素、甲基香兰素、乙基香兰素和乙基麦芽酚的测定。A quantitative method with stable isotope internal standard for the determination of vanillin,methyl vanillin,ethyl vanillin and ethyl maltol in vegetable oil by high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS)was established.Samples were extracted with acetonitrile-water and salted out by sodium chloride,separated by C18 chromatographic column,scanned in multiple reaction monitoring(MRM)mode,and quantitatively analyzed by internal standard method.Vanillin,methyl vanillin,ethyl vanillin and ethyl maltol were separated well and their calibration curves were linear in the concentration range of 2.0〜100.0 ng/mL.The relative standard deviation of the determination results was 2.46%〜10.1%(n=6),and the average recovery was 97.0%〜107.7%.The method used an internal standard to eliminate matrix effects and improved the data accuracy.It is suitable for the determination of vanillin,methyl vanillin,ethyl vanillin and ethyl maltol in vegetable oil.

关 键 词：香兰素 甲基香兰素 乙基香兰素 乙基麦芽酚 植物油 

分 类 号：TS207.3[轻工技术与工程—食品科学]