负载白藜芦醇的大麦醇溶蛋白/海藻酸丙二醇酯复合纳米颗粒的制备及表征  

Fabrication and Characterization of Resveratrol Loaded with Hordein / Propylene Glycol Alginate Composite Nanoparticles

作　　者：赵天瑜 唐荣 金雨楠 梅晓宏[1,2] Zhao Tianyu;Tang Rong;Jin Yunan;Mei Xiaohong(College of Food Science&Nutritional Engineering,China Agricultural University,Beijing 100083;Key Laboratory of Safety Assessment of Genetically Modified Organism(Food Safety),Ministry of Agriculture and Rural Affairs,Beijing 100083)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083 [2]农业农村部农业转基因生物安全评价(食用)重点实验室,北京100083

出　　处：《中国食品学报》2023年第9期89-98,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：2018年国家科技重大专项RNA干扰新型转基因产品食用安全评价关键技术与评价指南研究(2018ZX0801102B-002)。

摘　　要：以大麦醇溶蛋白(hordein)、海藻酸丙二醇酯(PGA)为原料,通过溶剂蒸发法制备大麦醇溶蛋白-海藻酸丙二醇酯复合纳米颗粒(Hordein/PGA),并对白藜芦醇进行包封。结果表明,随海藻酸丙二醇酯添加量的增加,Hordein/PGA的粒径由1654.9 nm逐渐下降至475.9 nm。同时Hordein/PGA的电位均为负值,并随海藻酸丙二醇酯添加量的增大由-3.62 mV下降至-8.04 mV。红外光谱结果显示:大麦醇溶蛋白与海藻酸丙二醇酯主要通过氢键、静电吸引及疏水相互作用结合。以Hordein/PGA1∶1对白藜芦醇进行包封,构建三元复合纳米颗粒(Hordein/PGA/Res),白藜芦醇的包封率可达87%。红外光谱和X射线衍射分析表明白藜芦醇与大麦醇溶蛋白、海藻酸丙二醇酯有效结合。Hordein/PGA/Res经85℃处理30 min后白藜芦醇保留率为88%,显著高于游离白藜芦醇保留率(73%);经6 h紫外光辐射处理后白藜芦醇保留率可提高约16%。In this study,Hordein/PGA composite nanoparticles were fabricated by solvent evaporation using hordein and PGA as materials,and resveratrol was encapsulated.The results indicated that the particle size of Hordein/PGA decreased from 1654.9 nm to 475.9 nm with the increase of PGA content.The potentials of Hordein/PGA were negative,and the value decreased from-3.62 mV to-8.04 mV with the increase of PGA content.Fluorescence spectra showed that hydrogen bonds,hydrophobic effects and electrostatic interactions contributed to the formation of Hordein/PGA composite nanoparticles.Resveratrol was encapsulated by Hordein/PGA1:1 to construct ternary composite nanoparticles(Hordein/PGA/Res),and the encapsulation rate of resveratrol reached 87%.Fourier transform infrared and X-ray diffraction demonstrated that resveratrol was effectively combined with hordein and PGA.The retention rate of resveratrol in Hordein/PGA/Res after heat treatment at 85℃for 30 min was 88%,which remarkably higher than the free resveratrol(73%).And the retention rate of resveratrol increased by about 16%after 6 h ultraviolet light treatment.

关 键 词：大麦醇溶蛋白 海藻酸丙二醇酯 白藜芦醇 复合纳米颗粒 

分 类 号：TS201.2[轻工技术与工程—食品科学]