不同蛋白酶对红娘鱼蛋白水解物的结构特性和生物活性的影响  

Effect of Different Proteases on Structural Characteristics and Biological Activity of Lepidotrigla microptera Protein Hydrolysates

作　　者：胡学佳 戴志远 金仁耀 张晓頔 董烨 Hu Xuejia;Dai Zhiyuan;Jin Renyao;Zhang Xiaodi;Dong Ye(Institute of Seafood,Zhejiang Gongshang University,Hangzhou 310035)

机构地区：[1]浙江工商大学海洋食品研究院,杭州310035

出　　处：《中国食品学报》2023年第10期81-89,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家重点研发计划项目(2018YFC0311205)。

摘　　要：以红娘鱼为原料,对木瓜蛋白酶、中性蛋白酶、复合蛋白酶、碱性蛋白酶和风味蛋白酶水解产物的结构特性、抗氧化及ACE抑制活性进行比较。采用高效液相色谱、紫外光谱、内源荧光光谱和傅里叶变换红外光谱测定红娘鱼蛋白在不同蛋白酶作用下的结构变化。通过测定水解产物ACE抑制率和抗氧化活性表征酶解产物的生物活性。与其它蛋白酶相比,碱性蛋白酶水解产物的低分子质量(<1000 u)占比和疏水氨基酸含量最高,分别为88.2%和200.5 mg/g,在结构表征中,碱性蛋白酶水解产物的β-转角和无规则卷曲也均高于其它蛋白酶,分别为69.68%和23%。同时碱性蛋白酶水解产物的抗氧化活性(·OH清除能力、DPPH自由基清除率和Fe2+螯合活性)和ACE抑制活性也是最高的,IC50值分别为1.88,1.92,1.76,0.77 mg/mL。结论:碱性蛋白酶是制备红娘鱼生物活性肽的最优蛋白酶。本研究为红娘鱼的多元化开发利用提供了理论支持。The objective of this study was to characterize the antioxidant activities,ACE inhibitory activity and functional properties of Lepidotrigla microptera which was hydrolyzed by papain,neutrase,protamex,alcalase and flavourzyme of hydrolysis.High performance liquid chromatograph,ultraviolet-visible(UV)absorption spectroscopy,intrinsic fluorescence spectroscopy and Fourier transform infrared spectroscopy(FTIR)were used to determine the structural changes during hydrolysis.The biological activity of the hydrolysate was characterized by measuring its antioxidant capacity and ACE inhibitory activity.Compared with the other groups,alcalase hydrolysate had the largest proportion of lowest molecular weight(<1000 u),and more hydrophobic amino acids,which were 88.2%and 200.5 mg/g,respectively.Structural characterization demonstrated that the surface hydrophobicity,random coil and β-turn of the hydrolysates were significantly higher than those of other groups,which were 69.68%,23%,respectively.Alcalase hydrolysate showed superior ACE inhibitory activity and antioxidant activity to other hydrolysates,the IC50 were 1.88,1.92,1.76,0.77 mg/mL,respectively.In conclusion,alcalase is the optimal protease for the preparation of Lepidotrigla microptera biological active peptide.This study provided data support and theoretical foundation for diversified development and utilization of Lepidotrigla microptera.

关 键 词：蛋白酶 红娘鱼 结构特性 抗氧化活性 ACE抑制活性 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]