基于蛋白质/肽组学的三文鱼物种溯源  

Traceability Analysis of Salmon Species Based on Proteomics/Peptidomics Analysis

作　　者：蒋冰雪 张晓梅 丁矛 王萍 张鸿伟 陈桂东 Jiang Bingxue;Zhang Xiaomei;Ding Mao;Wang Ping;Zhang Hongwei;Chen Guidong(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,Shandong;Technology Center of Qingdao Customs District,Qingdao 266114,Shandong;Qingdao Customs Risk Prevention and Control Bureau,Qingdao 266114,Shandong)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]青岛海关技术中心,山东青岛266114 [3]青岛海关风险防控分局,山东青岛266114

出　　处：《中国食品学报》2023年第10期273-283,共11页Journal of Chinese Institute Of Food Science and Technology

基　　金：海关总署科技计划项目(2020HK209);青海省重点研发与转化计划项目(2022NK132);青岛海关科研项目(QK201917,QK202101);山东省重点研发计划科技示范工程(2021SFGC0701)。

摘　　要：不同三文鱼在外观和物种亲缘关系上都十分相近,本研究旨在建立一种大西洋鲑鱼、大马哈鱼和虹鳟鱼的物种溯源方法。基于质谱鸟枪法蛋白质组学,在95%置信水平上共鉴定得到912个蛋白质。经过对不同三文鱼特征蛋白质相应肽段的检测特异性和物种特异性筛选分析,得到潜在的物种特征肽段生物标志物。通过多反应监测的靶向肽组学,进一步验证物种特征肽段生物标志物,得到了大西洋鲑鱼10条物种特征肽段、大马哈鱼2条物种特征肽段和虹鳟鱼3条物种特征肽段,并对市售的商品三文鱼产品进行了方法应用。结果显示,该方法操作方便、准确可靠、特异性好、适用性强,可在常规分析实验室对商品三文鱼产品进行有效的鉴别分析。Different salmons are from relative species and very similar in appearance.The aim of this study was to establish a species traceability method for Atlantic salmon,chum salmon and rainbow trout.A total of 912 proteins were identified at 95%confidence level based on mass spectrometry shotgun proteomics.Potential peptide biomarkers derived from different salmon unique proteins were assessed by detection specificity as well as species uniqueness,and the qualified ones were further validated by multiple reaction monitoring(MRM)based peptidomics,resulting in 10 species-specific peptides for Atlantic salmon,2 species-specific peptides for chum salmon and 3 species-specific peptides for rainbow trout.The results demonstrated that the proposed approach is simple,accurate,reliable,specific and robust,and can be effectively used for the identification and analysis of commercial salmon products in routine analytical laboratories.

关 键 词：大西洋鲑鱼 大马哈鱼 虹鳟鱼 特征肽段 质谱 蛋白质组学 物种鉴别 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]