ε-聚赖氨酸生产菌育种研究进展  

Advances in Strain Improvement for the Production ofε-Poly-L-lysine

作　　者：孙子龙 扶教龙[1] 王月 周运峰 李博彦 钱玮[1] 白净 鞠鑫 李良智[1] SUN Zilong;FU Jiaolong;WANG Yue;ZHOU Yunfeng;LI Boyan;QIAN Wei;BAI Jing;JU Xin;LI Liangzhi(School of Chemical and Life Sciences,Suzhou University of Science and Technology,Suzhou 215009,China)

机构地区：[1]苏州科技大学化学与生命科学学院,江苏苏州215009

出　　处：《食品科学》2023年第23期231-239,共9页Food Science

基　　金：国家自然科学基金面上项目(21676173);苏州市科技计划项目(SNG2017053);苏州科技学院科研基金资助项目(XKZ201411)。

摘　　要：ε-聚赖氨酸(ε-poly-L-lysine,ε-PL)是一种含25~35个L-赖氨酸残基的新型生物聚合物,由ε-NH2和α-COOH脱水缩合形成。其具有热稳定性、可食性、水溶性、可降解性、抑菌谱广及无毒等优异性能,作为一种安全的食品防腐剂,在日本、韩国、美国、中国和一些其他国家得以应用。ε-PL通常由白色链霉菌(Streptomyces albulus)发酵生产,对生产菌进行育种改造是提高ε-PL生产和降低成本的关键方法之一。目前,研究者们已通过理化诱变、核糖体工程、基因组重排、基因工程等方法对ε-PL生产菌进行了大量育种研究,且得到了一些高产菌株。本文首先简要介绍ε-PL生物合成机理,并从上述几个方面着重介绍ε-PL生产菌育种研究进展,然后概述ε-PL发酵生产工艺,最后对相关研究进行展望,以期为ε-PL微生物育种和绿色生物制造提供借鉴与参考。ε-Poly-L-lysine(ε-PL)is a novel biopolymer consisting of 25–35 L-lysine residues,which is formed by the dehydration condensation ofε-NH2 andα-COOH.ε-PL possesses many excellent characteristics,such as antimicrobial activity,edibility,water solubility,biodegradability,thermostability and nontoxicity.As a natural and safe food preservative,ε-PL possesses many excellent advantages such as thermal stability,edibility,water solubility,degradability,and broad-spectrum antibacterial activity and has been successfully utilized in Japan,South Korea,the United States,China and other countries.ε-PL is usually produced by fermentation with Streptomyces albulus,and improvingε-PL-producing stains is crucial for enhancingε-PL production and reducing costs.At present,researchers have obtained microbial strains capable of producing high levels ofε-PL by using physicochemical mutagenesis,ribosome engineering,genome shuffling,genetic engineering and other methods.This review introduces the mechanism ofε-PL biosynthesis and recent progress in strain improvement for the production ofε-PL,and gives an overview of the fermentation process ofε-PL.Finally,this review concludes with an outlook on future research directions.We hope that this review can help promote strain improvement for green biological manufacturing ofε-poly-L-lysine.

关 键 词：Ε-聚赖氨酸 育种 核糖体工程 基因工程 白色链霉菌 

分 类 号：TS201.3[轻工技术与工程—食品科学]