一株植物乳杆菌的分离鉴定及其对红曲霉产红曲色素的影响  

Isolation and Identification of Lactobacillus plantarum and Its Effect on Pigment Production by Monascus

作　　者：伍忠玲 高红艳[1] 刘振民[1] WU Zhongling;GAO Hongyan;LIU Zhenmin(Shanghai Engineering Research Center of Dairy Biotechnology,State Key Laboratory of Dairy Biotechnology,Dairy Research Institute,Bright Dairy and Food Co.Ltd.,Shanghai 200436,China)

机构地区：[1]乳业生物技术国家重点实验室,上海乳业生物工程技术研究中心,光明乳业股份有限公司乳业研究院,上海200436

出　　处：《乳业科学与技术》2023年第5期1-6,共6页JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY

基　　金：上海市国资委企业创新发展和能级提升项目(2022013)。

摘　　要：以红腐乳为原料分离到1株乳酸菌菌株BD-1,研究BD-1菌株与BD-1菌株发酵上清液对紫色红曲霉(Monascus purpureus)ATCC 16365菌株产红曲色素(Monascus pigments,MPs)的影响。结果表明:通过革兰氏染色、生理生化和16S rRNA基因测序鉴定该BD-1菌株为植物乳杆菌(Lactobacillus plantarum);通过设置0.2%、0.4%、0.6%、0.8%、1.0%的BD-1菌株活菌接种量及ATCC 16365菌株培养0、24、48 h后接种BD-1菌株分析发现,相比于对照组,在ATCC 16365菌株培养24 h后加入0.6%BD-1菌株,胞外红曲色素(extracellular Monascus pigments,EMPs)色价及合成促进率显著提高,分别达(6.24±0.05)U/mL与(146.55±7.84)%,胞内红曲色素(intracellular Monascus pigments,IMPs)色价及合成促进率显著提高,分别达(288.14±6.82)U/g与(16.07±0.30)%;此外,相比于对照组,BD-1菌株发酵上清液加入ATCC 16365菌株培养,EMPs色价及合成促进率显著提高,分别达(4.20±0.08)U/mL与(29.85±5.40)%。综上,0.6%BD-1菌株活菌接种量为产MPs的最佳接种量。A lactic acid bacteria(LAB)strain BD-1 was isolated from red fermented bean curd,and the effect of BD-1 and its fermentation supernatant on the production of Monascus pigments(MPs)by Monascus purpureus ATCC 16365 was studied.The results showed that BD-1 was identified as Lactobacillus plantarum through Gram staining,physiological and biochemical profiles and 16S rRNA gene sequencing.Five different inoculum sizes of 0.2%,0.4%,0.6%,0.8%and 1.0%were tested for strain BD-1 and ATCC 16365 was cultured for 0,24 or 48 h.It was found that adding 0.6%BD-1 to the fermented medium with ATCC 16365 after culture for 24 h markedly increased the color value and synthesis of extracellular Monascus pigments(EMPs)and intracellular Monascus pigments(IMPs).Under this condition,the color values of EMPs and IMPs were(6.24±0.05)and(288.14±6.82)U/mL,and their synthesis was increased by(146.55±7.84)%and(16.07±0.30)%compared with the control group,respectively.In addition,adding the fermentation supernatant of BD-1 to ATCC 16365 also remarkably increased the color value and synthesis of EMPs,which were increased to 4.20 U/mL and by(29.85±5.40)%compared to the control group,respectively.In summary,the optimal inoculum size of BD-1 for MPs production was 0.6%.

关 键 词：乳酸菌分离 植物乳杆菌 红曲霉 红曲色素 

分 类 号：TS201.3[轻工技术与工程—食品科学]