植物乳杆菌固体发酵对复方中药中有效成分含量的影响  

Effect of solid fermentation of Lactobacillus plantarum on the contents of effective components in compound Chinese medicine

作　　者：侯楠楠[1] 谢全喜[1] 王俊贤 王倩 王梅 谷巍[1] HOU Nannan;XIE Quanxi;WANG Junxian;WANG Qian;WANG Mei;GU Wei(Shandong Key Laboratory of Animal Microecological Preparations,Shandong BaoLai-LeeLai bioengineering Co.,Ltd.,Tai'an 271000,China)

机构地区：[1]山东宝来利来生物工程股份有限公司,山东省动物微生态制剂重点实验室,山东泰安271000

出　　处：《中国酿造》2024年第2期221-225,共5页China Brewing

基　　金：泰安市科技发展计划(201540701)。

摘　　要：该研究采用植物乳杆菌(Lactobacillus plantarum)BLCC2-0015、BLCC2-0126和BLCC2-0410固体发酵复方中药(黄芪、甘草和益母草等),测定发酵过程中复方中药的pH、微生物数量及总酸、有机酸、粗多糖等有效成分含量的变化,评价其对复方中药有效成分含量的影响。结果表明,植物乳杆菌发酵可降低复方中药的pH值,提高乳杆菌活菌数,抑制大肠杆菌(Escherichia coli)及霉菌的生长,并提高总酸、乳酸、苹果酸、粗多糖、阿魏酸及甘草次酸的含量。其中,植物乳杆菌BLCC2-0410发酵效果最好,发酵72 h时,复方中药的pH为4.32;乳杆菌活菌数为4.53×10^(9)CFU/g,大肠杆菌及霉菌未检出;总酸、乳酸、苹果酸、粗多糖、阿魏酸和甘草次酸含量均显著升高(P<0.05),分别为27.82 g/kg、19.25 g/kg、4.06 g/kg、5.83%、42.00 mg/kg和517.9 mg/kg。Compound Chinese medicine(Astragalus,licorice and Leonurus japonicus,etc.)were prepared with solid state fermentation by Lactobacillus plantarum BLCC2-0015,BLCC2-0126 and BLCC2-0410.The pH,microbial quantities and the contents of effective components such as total acid,organic acid and crude polysaccharide were determined during the fermentation process,and the influence on the contents of effective components in compound Chinese medicine was evaluated.The results showed that L.plantarum fermentation could decrease the pH,increase the viable L.plantarum counts,inhibit the growth of Escherichia coli and molds,and increase the contents of total acid,lactic acid,malic acid,crude polysaccharide,ferulic acid and glycyrrhetinic acid.Among them,fermentation effect of L.plantarum BLCC2-0410 was the optimal,and the pH of the compound Chinese medicine was 4.32 after fermentation for 72 h.The viable L.plantarum counts was 4.53×10^(9) CFU/g,and E.coli and molds were not detected.The contents of total acid,lactic acid,malic acid,crude polysaccharide,ferulic acid and glycyrrhetinic acid significantly increased(P<0.05),which were 27.82 g/kg,19.25 g/kg,4.06 g/kg,5.83%,42.00 mg/kg and 517.9 mg/kg,respectively.

关 键 词：植物乳杆菌 复方中药 固体发酵 乳酸 阿魏酸 甘草次酸 

分 类 号：Q939.11[生物学—微生物学]