基于改变相比法结合HS-GC-MS测定烟丝基质中酯类单体香料的固-气分配系数  

Determing solid-gas partition coefficients of monomer ester flavors in cut tobacco matrix by changing comparison method with HS-GC-MS

作　　者：赵佳明 付英杰 罗曾源 席辉[1] 卢斌斌[1] 张帅[2] 陈帅伟 王增瑜 宗永立[1] 李军[2] 孙世豪[1] ZHAO Jiaming;FU Yingjie;LUO Zengyuan;XI Hui;LU Binbin;ZHANG Shuai;CHEN Shuaiwei;WANG Zengyu;ZONG Yongli;LI Jun;SUN Shihao(Zhengzhou Tobacco Research Institute of CNTC,Zhengzhou 450001,China;China Tobacco Shandong Industrial Co.,Ltd,Jinan 250014,China)

机构地区：[1]中国烟草总公司郑州烟草研究院,郑州450001 [2]山东中烟工业有限责任公司技术中心,济南市250014

出　　处：《烟草科技》2024年第3期1-10,共10页Tobacco Science & Technology

基　　金：国家烟草专卖局创新平台科研活动稳定支持专项经费资助;山东中烟工业有限责任公司科技项目“烟用香料香气物质释放规律研究”(432023DG0240)。

摘　　要：为了借助固-气分配系数对烟草基质中外加风味成分的释放行为进行深入剖析,基于改变相比法结合HS-GC-MS分析方法,建立了一种适用于烟丝基质中单体香料分配系数测定的PRV-HS-GC-MS方法,采用PRV-HS-GC-MS法测定了6种酯类香料在烟丝基质(固相)和气相中的分配系数(K_(cut/air))。结果表明:(1)在90~110℃条件下6种酯类香料K_(cut/air)的相对标准偏差(RSD)为0.92%~9.14%,所建立的方法精密度高,可重复性好,便捷准确。(2)烟丝中6种酯类单体香料的K_(cut/air)值与温度呈负相关关系;以低温区域内K_(cut/air)值所建立的预测方程可对难以测定的高温区域下的K_(cut/air)值进行预测,90~110℃时K_(cut/air)实际测定值与预测值的相对误差在1.04%~5.32%之间,预测效果较好。(3)K_(cut/air)值可以作为烟丝基质中单体香料释放性能参数,量化表征单体香料释放行为的差异,为烟用单体香料释放行为提供新的研究途径。In order to analyze in depth the release behavior of added flavor components in tobacco matrix using the solid-gas partition coefficient,a PRV-HS-GC-MS method was developed to determine the partition coefficients of monomeric flavors in cut tobacco matrix based on the changing comparison method combined with HS-GC-MS analysis.The partition coefficients(K_(cut/air))of six ester flavors in cut tobacco matrix(solid phase)and gas phase were determined by the PRV-HS-GC-MS method.The results showed that:1)The relative standard deviation(RSD)of K_(cut/air) of the six ester flavors at 90-110℃ranged from 0.92%to 9.14%.The developed method was convenient and accurate with good repeatability.2)The K_(cut/air) values of the six monomer ester flavors in cut tobacco were negatively correlated with temperature.The prediction equations established on the basis of K_(cut/air) values in low-temperature regions could predict K_(cut/air) values that were difficult to determine in high-temperature regions.The relative errors between the actually determined and predicted K_(cut/air) values at 90-110℃were between 1.04%and 5.32%,indicating good prediction performance.3)The K_(cut/air) values could be used as a parameter for the release performance of monomer flavors in cut tobacco matrix to quantitatively characterize the differences in monomer flavor release behavior,and it provided a new strategy to study the release behavior of tobacco monomer flavors.

关 键 词：酯类化合物 单体香料 烟草基质 分配系数 改变相比法 静态顶空气相色谱 

分 类 号：TS411.1[农业科学—烟草工业]