富锗木耳菌丝体不同溶剂提取物的体外抗氧化能力  

Antioxidant Capacity of Different Solvent Extracts from Ge-riched Auricularia auricula Mycelium in Vitro

作　　者：朱雨馨 王文斌 王佳婧 朱蕴兰[1] 张翼飞 徐薛 戴永琪 ZHU Yuxin;WANG Wenbin;WANG Jiajing;ZHU Yunlan;ZHANG Yifei;XU Xue;DAI Yongqi(College of Food and Biology Engineering,Xuzhou University of Technology,Jiangsu Research Center of Food and Biology Process Engineering Technology,Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe,Xuzhou 221018,China;Nanjing University of Chinese Medicine,Nanjing 210046,China)

机构地区：[1]徐州工程学院食品与生物工程学院,江苏省食品生物加工工程技术研究中心,江苏省食品资源开发与质量安全重点建设实验室,江苏徐州221018 [2]南京中医药大学,江苏南京210046

出　　处：《食品工业科技》2024年第8期21-28,共8页Science and Technology of Food Industry

基　　金：徐州工程学院大学生创新训练计划项目(XCX2023234)。

摘　　要：为探索富锗木耳菌丝体不同溶剂提取物的体外抗氧化能力,以富锗木耳菌丝体为材料,通过水、70%乙醇和乙酸乙酯对富锗木耳菌丝体进行了物质提取,比较了不同提取物对超氧阴离子自由基、羟自由基和DPPH自由基的清除能力。采用隶属函数值评价了不同溶剂提取物的综合抗氧化能力,并对物质含量与抗氧化能力进行了相关性分析。结果表明:富锗木耳菌丝体有机锗含量为542.83μg/g。不同溶剂提取物的提取率和物质含量均不同,水和乙醇提取物的提取率较高,水提取物中多糖含量最多,乙酸乙酯提取物中多酚和黄酮含量最多。不同溶剂提取物对自由基均有一定的清除能力,且随着提取物浓度的增加而增加。水提取物对超氧阴离子自由基清除率最强,当提取物浓度为9 mg/mL时,清除率最高为43.32%。70%乙醇提取物对羟自由基和DPPH自由基的清除效果最好,当提取物浓度为9 mg/mL时,清除率分别为70.10%和76.99%。多糖、多酚和黄酮在清除自由基中均发挥不同的作用,70%乙醇提取物的综合抗氧化能力最强。多糖、多酚和黄酮含量均与DPPH自由基清除率有显著(P<0.05)正相关。70%乙醇提取物中多酚含量与羟自由基清除率有显著(P<0.05)正相关。多糖、多酚及黄酮对超氧阴离子自由基的清除率均有显著(P<0.05)影响。富锗木耳菌丝体含有较高含量的有机锗,提取物对自由基具有一定的清除能力,具有较好的抗氧化性能。本研究对开发富锗木耳功能食品具有一定指导意义。In order to explore the antioxidant ability of different solvent extracts of Ge-riched Auricularia auricular mycelium in vitro,the Ge-riched A.auricular mycelium was extracted by water,70%ethanol and ethyl acetate.The scavenging ability of different extracts on superoxide anion free radical,hydroxyl free radical and DPPH free radical were compared.The comprehensive antioxidant capacity of different solvent extracts was evaluated by subordinate function value,and the correlation between substance content and antioxidant capacity were analyzed.The results showed that the content of organic Ge in Ge-riched A.auricular mycelium was 542.83μg/g.The extraction rate and substance content of different solvent extracts were different,the extraction rate of water extract and ethanol extract was higher,the polysa-ccharide content of water extract was the highest,and the polyphenol and flavonoid content of ethyl acetate extract was the highest.Different solvent extracts had certain scavenging ability on free radicals,and the extract concentration increased with the increase.Water extract had the strongest scavenging rate of superoxide anion free radical,when the extract concentration was 9 mg/mL,the highest clearance rate was 43.32%.70%ethanol extract had the best scavenging effect on hydroxyl free radical and DPPH free radical.When the extract concentration was 9 mg/mL,the scavenging rate was 70.10%and 76.99%,respectively.Polysaccharides,polyphenols and flavonoids all played different roles in scavenging free radicals,and ethanol extract had the strongest comprehensive antioxidant capacity.The contents of polysaccharides,polyphenols and flavonoids were significant(P<0.05)positively correlated with DPPH free radical clearance.The content of polyphenol in 70%ethanol extract was significant(P<0.05)positively correlated with the scavenging rate of hydroxyl radical.The scavenging rate of superoxide anion radical was significantly(P<0.05)affected by polysaccharides,polyp-henols and flavonoids.Ge-riched A.auricular mycelium contai

关 键 词：木耳 不同溶剂 提取物 有机锗 抗氧化活性 多糖 多酚 黄酮 

分 类 号：TS201.1[轻工技术与工程—食品科学]