腐乳混合发酵剂配方优化  

Optimization of Curd Fermentation Mix Formulation

作　　者：杨润 任淑娣 陈中爱 刘丽静[1] 许家威 胡永金[1] YANG Run;REN Shudi;CHEN Zhongai;LIU Lijing;XU Jiawei;HU Yongjin(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201)

机构地区：[1]云南农业大学食品科学技术学院,昆明650201

出　　处：《食品工业》2024年第3期95-100,共6页The Food Industry

基　　金：科技人才与平台计划(202105AF150049);云南省高校食品微生物资源与利用重点试验室(云教发[2018]135号)。

摘　　要：单菌种发酵腐乳存在酶系不全、风味单一等问题。此研究选用毛霉M-T和毛霉40899优势双菌种制备发酵剂,通过对发酵剂水分添加量、pH、菌种添加量以及菌种比例等工艺参数研究,确定最优的发酵剂配方。采用优化后的发酵剂发酵腐乳,测定72 h内蛋白酶活力、总酸以及氨基酸态氮的变化。结果表明:发酵剂的最佳水分添加量为培养基质的111%,pH为3.22,菌液比为0.69,发酵剂成品中霉菌数为8.35 lg (CFU/g)。发酵剂培养的毛坯在72 h时蛋白酶活力能达到37.25μg/g;与传统腐乳前发酵时期的总酸含量对比,发酵剂发酵的腐乳总酸含量上升得稍快,氨基酸态氮含量由0 h的0.004 g/100 g上升到72 h的0.056 g/100 g。该发酵剂的发酵性能良好,为腐乳产业规模化生产提供了参考。Single strain fermented curd has problems such as incomplete enzyme system and single flavour.In this study,Trichoderma M-T and Trichoderma 40899 were used to prepare the fermenter,and the optimal fermenter formulation was determined by studying the process parameters such as the amount of water added to the fermenter,pH,the amount of strains added to the fermenter,and the ratio of strains.The optimized fermenter was used to ferment curd,and the changes of protease activity,total acid and amino acid nitrogen were determined within 72 h.The results showed that the fermenter was suitable for the fermentation of fermented curd,but it was not suitable for the fermentation of curd.The results showed that the optimum moisture addition of the fermenter was 111% of the culture substrate,the pH was 3.22,the bacterial-liquid ratio was 0.69,and the mould count in the finished fermenter was 8.35 lg(CFU/g).The protease activity of the fermenter-cultured blanks could reach 37.25 μg/g at 72 h.Compared with the total acid content during the pre-fermentation period of traditional curd,the total acid content of the fermenter-fermented curd rose slightly faster,and the content of amin acid nitrogen increased from 0.004g/100 g at 0 h to 0.056 g/100 g at 72 h.The fermentation performance of the fermenter was good,and it provided a reference for the large-scale production of curd in the curd industry.The fermentation performance of this fermenter is good,which provides a reference for the large-scale production of curd industry.

关 键 词：腐乳 毛霉 混合发酵剂 工艺优化 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程] TS201.3[轻工技术与工程—食品科学与工程]