黄褐假单胞菌Y11产胞外多糖发酵条件优化及其抗氧化活性研究  

Optimization of fermentation conditions for extracellular polysaccharide production by Pseudomonas syringae Y11 and its antioxidant activity

作　　者：龙丹丹 叶淑红[1] 燕欣悦 王琛郴 崔艳平 张彧[1] LONG Dandan;YE Shuhong;YAN Xinyue;WANG Chenchen;CUI Yanping;ZHANG Yu(School of Food,Dalian University of Technology,Dalian 116034,China)

机构地区：[1]大连工业大学食品学院,辽宁大连116034

出　　处：《中国酿造》2024年第4期115-122,共8页China Brewing

基　　金：国家自然科学基金(32171836);辽宁省自然科学基金(2020-M ZLH-37);辽宁省教育厅科学研究经费项目(面上项目)(LJKZ0538)。

摘　　要：为提高黄褐假单胞菌(Pseudomonas syringae)液态发酵产胞外多糖(EPS)量,以胞外多糖产量为评价指标,通过单因素及响应面试验对菌株发酵产胞外多糖条件进行优化,并对其胞外多糖的抗氧化性进行研究。结果表明,黄褐假单胞菌最佳产胞外多糖条件为麦芽糖44 g/L、大豆蛋白胨11 g/L、磷酸二氢钠8 g/L、接种量4%、转速160 r/min、发酵温度28℃、发酵时间72 h、初始pH 7.0。在此优化条件下,胞外多糖的产量为4.56 g/L,是优化前的3.7倍。抗氧化性研究结果表明,在一定浓度范围内,EPS对DPPH自由基、ABTS自由基、羟自由基、超氧阴离子自由基具有较好的清除活性,当胞外多糖质量浓度为2.5 mg/mL时,EPS对DPPH自由基、ABTS自由基、羟自由基、超氧阴离子自由基的清除能力分别为(70.83±1.80)%、(45.00±1.73)%、(65.83±1.80)%和(44.6±2.11)%,说明黄褐假单胞菌液态发酵产胞外多糖具有一定的抗氧化活性。In order to improve the yield of extracellular polysaccharide(EPS)by liquid fermentation of Pseudomonas fulva,using extracellular polysaccharide yield as an evaluation index,the EPS production conditions of the strain were optimized by single factor and response surface tests,and the antioxidant properties of the EPS were investigated.The results showed that the optimal conditions for the production of extracellular polysaccharides by P.fulva were maltose 44 g/L,soybean peptone 11 g/L,sodium dihydrogen phosphate 8 g/L,inoculum 4%,rotational speed 160 r/min,fermentation temperature 28℃,time 72 h,and initial pH 7.0.The yield of EPS under the optimal conditions was 4.56 g/L,which was 3.7 times that of before optimization.The results of antioxidant activities showed that EPS had good scavenging activity on DPPH radicals,ABTS radicals,hydroxyl radicals,and superoxide anion radicals in a certain concentration range.When the mass concentration of EPS was 2.5 mg/ml,the scavenging ability of EPS on DPPH radicals,ABTS radicals,hydroxyl radicals,and superoxide anion radicals was(70.83±1.80)%,(45.00±1.73)%,(65.83±1.80)%,and(44.6±2.11)%,respectively,which indicated that the EPS produced by the liquid fermentation of P.fulva had certain antioxidant activity.

关 键 词：黄褐假单胞菌 胞外多糖 发酵条件 响应面法 抗氧化性 

分 类 号：TS262.6[轻工技术与工程—发酵工程]