核桃多肽结构表征及抗氧化活性  

Structural Characterization and Antioxidant Activity of Walnut Peptides

作　　者：张子杰 田益玲[2] 夏君霞 马爱进 ZHANG Zijie;TIAN Yiling;XIA Junxia;MA Aijin(School of Food and Health,Beijing Technology and Business University,Beijing 100048,China;College of Food Science and Technology,Hebei Agricultural University,Baoding 071001,China;Hebei Yangyuan Zhihui Beverage Co.Ltd.,Hengshui 053000,China)

机构地区：[1]北京工商大学食品与健康学院,北京100048 [2]河北农业大学食品科技学院,河北保定071001 [3]河北养元智汇饮品股份有限公司,河北衡水053000

出　　处：《食品科学》2024年第9期1-8,共8页Food Science

基　　金：“十四五”国家重点研发计划重点专项(2022YFD1600402)。

摘　　要：以核桃粕为原料,复合酶酶解制备核桃多肽,并通过超滤分离获得不同分子质量的核桃多肽,进行结构表征和实验分析,探究核桃多肽的抗氧化活性及其对氧化损伤HepG2细胞的保护作用。结果表明,分子质量<1 kDa核桃蛋白水解产物抗氧化活性最强,其羟自由基清除能力的半抑制浓度(half-maximal inhibitory concentration,IC_(50))值为11.47 mg/mL、1,1-二苯基-2-三硝基苯肼自由基清除能力的IC_(50)值为35.67μg/mL、2,2’-联氮双(3-乙基苯并噻唑啉-6-磺酸)阳离子自由基清除能力的IC_(50)值为49.72μg/m L。同时,<1 kDa核桃多肽组分能够减少Hep G2细胞内活性氧含量,提高超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶和谷胱甘肽过氧化物酶活力。核桃多肽在225 nm波长处有最大吸收峰。核桃多肽形状不规则、表面光滑、结构致密,有大量粗糙纹路和孔隙。核桃多肽的二级结构中无规卷曲(36.5%)和β-折叠(36.6%)相对含量最高。综上,<1 kDa核桃多肽对氧化损伤Hep G2细胞具有保护作用。This study prepared and structurally characterized peptides with different molecular masses from walnut meal protein by enzymatic hydrolysis with a mixture of two proteases followed by ultrafiltration,and it also explored the antioxidant activities of walnut peptides and their protective effects on oxidative damage in HepG2 cells.The results showed that the antioxidant activity of walnut protein hydrolysate(WPH)with molecular mass<1 kDa was the strongest,with half-maximal inhibitory concentration(IC_(50))of 11.47,35.67 and 49.72 mg/mL for hydroxyl radical,1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)radical cation scavenging capacity,respectively.Moreover,the<1 kDa walnut peptide fraction could reduce the reactive oxygen species(ROS)content and increase the superoxide dismutase(SOD),catalase(CAT)and glutathione reductase(GSH-Rx)and glutathione peroxidase(GSH-Px)activity in HepG2 cells.The walnut peptide was irregular in shape and had a smooth surface and dense structure with numerous rough patterns and pores.The walnut peptide had a maximum absorption peak at 225 nm wavelength.The most abundant secondary structures of the walnut peptide were random coil(36.5%)andβ-sheet(36.6%).These results indicate that the<1 kDa walnut peptide has a protective effect against oxidative damage in HepG2 cells.

关 键 词：核桃粕 多肽 抗氧化活性 结构表征 

分 类 号：TS201.2[轻工技术与工程—食品科学]