壳聚糖-单宁酸涂膜对叫化鸡微生物多样性的影响  

effect of Chitosan-tannic Acid Coating on the Microbial Diversity of Jiaohuaji Chicken

作　　者：张晓千 苏巍 刘小莉[1] 胡根河 周剑忠[1] 范琳琳[1] ZHANG Xiaoqian;SU Wei;LIU Xiaoli;HU Genhe;ZHOU Jianzhong;FAN Linlin(Institute of Agro-product Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China;Changshu Wangsi Food Company Limited,Changshu 215500,China)

机构地区：[1]江苏省农业科学院农产品加工研究所,江苏南京210014 [2]上海海洋大学食品学院,上海201306 [3]常熟市王四食品有限公司,江苏常熟215500

出　　处：《食品科学》2024年第11期128-135,共8页Food Science

基　　金：江苏省重点研发计划项目(BE2020387)。

摘　　要：为探讨壳聚糖-单宁酸涂膜对叫化鸡贮藏期间品质的影响,本研究重点分析经过涂膜处理前后叫化鸡的微生物多样性,并分析贮藏期间菌落总数、pH值、硫代巴比妥酸反应物(thiobarbituric acid reactive substances,TBARS)值和总挥发性盐基氮(total volatile basic nitrogen,TVB-N)值的变化。高通量测序分析结果表明,芽孢杆菌属(Bacillus)、链霉菌属(Streptophyta)和普雷沃氏菌属(Prevotella)是叫化鸡贮藏期间的主要菌属,芽孢杆菌属相对丰度在贮藏第5天(37℃)增加到99.04%,为优势菌群。涂膜处理后芽孢杆菌属相对丰度降低了13.53%,普雷沃氏菌属和其他菌属丰度上升。此外,菌落总数、理化指标分析结果表明,涂膜处理后的叫化鸡菌落总数8 d(4℃)内保持在5.0(lg(CFU/g))以下,pH值显著降低,TBARS值和TVB-N值在贮藏结束(4℃)时较空白对照组分别降低了22.28%和28.15%。壳聚糖-单宁酸涂膜处理改变了贮藏过程中叫化鸡菌群组成,一定程度上抑制了腐败菌生长,延缓脂质蛋白质氧化,延长了叫化鸡保质期。In order to explore the effect of chitosan-tannic acid coating on the quality of Jiaohuaji chicken during storage,the microbial diversity of Jiaohuaji chicken with and without the coating treatment was evaluated.The changes in total bacterial count(TBC),pH,thiobarbituric acid reactive substances(TBARS)and total volatile basic nitrogen(TVB-N)values during storage were analyzed as well.The results of high-throughput sequencing analysis showed that Bacillus,Streptophyta,and Prevotella were the dominant genera during the storage period of Jiaohuaji chicken.The abundance of Bacillus increased to 99.04%on the fifth day of storage(37℃),indicating it to be dominant.After the coating treatment,the abundance of Bacillus in Jiaohuaji chicken decreased by 13.53%whereas the abundance of Prevotella and other genera increased.In addition,the results of microbial and physicochemical analysis showed that the coating treatment kept the TBC below 5.0(lg(CFU/g))for 8 days(4℃),significantly reduced the pH,and decreased the TBARS and TVB-N values by 22.28%and 28.15%,respectively at the end of storage(4℃)compared with the blank control group.To sum up,the chitosan-tannic acid coating treatment changed the composition of the bacterial flora,inhibited the growth of spoilage bacteria to some extent,delayed lipid and protein oxidation,and prolonged the shelf life of Jiaohuaji chicken.

关 键 词：壳聚糖 单宁酸 叫化鸡 微生物多样性 保鲜 

分 类 号：TS251[轻工技术与工程—农产品加工及贮藏工程]