高效液相色谱-蒸发光散射法测定油莎豆块茎发育过程中的水溶性糖  

Dertermination of water-soluble sugars of Cyperus esculentus L.tubers in the growth period by HPLC-ELSD

作　　者：杨亚琴[1] 余明霞 冯慧慧 李春鑫[2] 于美琴 郝学飞[1] YANG Ya-qin;YU Ming-xia;FENG Hui-hui;LI Chun-xin;YU Mei-qin;HAO Xue-fei(Institute of Agro-product Quality and Safety,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China;Industrial Crop Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002,Henan,China)

机构地区：[1]河南省农业科学院农产品质量安全研究所,河南郑州450002 [2]河南省农业科学院经济作物研究所,河南郑州450002

出　　处：《粮食与油脂》2024年第6期153-157,162,共6页Cereals & Oils

基　　金：河南省农业科学院优秀青年科技基金计划项目(2022YQ29);河南省农业科学院新兴学科发展专项(2023XK01);河南省重点研发与推广(科技攻关)专项(232102110188)。

摘　　要：基于高效液相色谱-蒸发光散射法(HPLC-ELSD)建立油莎豆块茎发育过程中水溶性糖(果糖、葡萄糖、蔗糖)含量的检测方法。以乙腈∶水(体积比75∶25)为流动相,流速为1.0 mL/min,并将蒸发光散射检测器条件优化为氮气压力206.8 kPa、漂移管温度65℃。结果表明:3种水溶性糖在0.10~20.0 mg/mL质量浓度范围内线性关系良好(r>0.999),检出限为0.04~0.10 g/100 g,回收率为96.0%~100.2%,相对标准偏差(RSD)为1.00%~7.32%(n=6)。在油莎豆块茎发育过程中,其果糖含量为0.376~0.692 g/100 g,葡萄糖含量为0.366~0.787 g/100 g,两者呈显著正相关(P<0.01);蔗糖含量为5.49~24.2 g/100 g,呈先降低后总体升高的趋势。所建方法灵敏度高、重现性好,适用于油莎豆块茎发育过程中水溶性糖的测定。A detection method was established for determining water-soluble sugar(fructose,glucose,sucrose)content of Cyperus esculentus L.tubers in the growth period based on high performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD).Acetonitrile:water(volume ratio 75:25)was used as the mobile phase,with a flow rate of 1.0 mL/min.The conditions of ELSD were optimized to nitrogen pressure of 206.8 kPa and drift tube temperature of 65℃.The results showed that the three water-soluble sugars had good linear relationships within the mass concentration range of 0.10-20.0 mg/mL(r>0.999),with detection limits of 0.04-0.10 g/100 g,recovery rates of 96.0%-100.2%,and relative standard deviation(RSD)of 1.00%~7.32%(n=6).For Cyperus esculentus L.tubers in the growth period,the fructose content was 0.376-0.692 g/100 g,the glucose content was 0.366-0.787 g/100 g,which showed a significant positive correlation(P<0.01).The sucrose content was 5.49-24.2 g/100 g,which showed a trend of decreasing first and then overall increasing.The established method had high sensitivity and good reproducibility,and was suitable for the determination of water-soluble sugars in Cyperus esculentus L.tubers in the growth period.

关 键 词：高效液相色谱 蒸发光散射 油莎豆 块茎 水溶性糖 

分 类 号：TS207.3[轻工技术与工程—食品科学]