短乳杆菌M-10产胞外多糖的发酵工艺优化  

Optimization of fermentation process of exopolysaccharide production by Levilactobacillus brevis M-10

作　　者：王琪[1] 李根 刘鹏帆 彭佳伟 秦文君[3] 蔡瑾[4] WANG Qi;LI Gen;LIU Pengfan;PENG Jiawei;QIN Wenjun;CAI Jin(School of Life Science,Shanxi University,Taiyuan 030031,China;Jiangxi Lidu Liquor Co.,Ltd.,Nanchang 331725,China;Nutritional Department,Shanxi Traditional Chinese Medical Hospital,Taiyuan 030012,China;Institute of Applied Chemistry,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西大学生命科学学院,山西太原030631 [2]江西李渡酒业有限公司,江西南昌331725 [3]山西省中医药研究院营养科,山西太原030012 [4]山西大学应用化学研究所,山西太原030006

出　　处：《中国酿造》2024年第6期162-167,共6页China Brewing

基　　金：山西省自然科学研究面上项目(20210302123464)。

摘　　要：该研究以分离自晋西北酸粥、并能产生胞外多糖(EPS)的短乳杆菌(Levilactobacillus brevis)M-10为研究对象,采用MRS培养基,在优化碳源、氮源的基础上,通过单因素试验和响应面试验,对短乳杆菌M-10产EPS的发酵工艺进行优化。结果表明,发酵温度对短乳杆菌M-10产EPS影响极显著(P<0.01),接种量对短乳杆菌M-10产EPS影响显著(P<0.05)。最佳发酵工艺为发酵温度36℃,发酵时间45 h,初始pH值6.2,接种量1.0%(V/V)。在此优化条件下,EPS产量为(2.54±0.13)g/L,比优化前提高了2.42倍。Using exopolysaccharide(EPS)producing Levilactobacillus brevis M-10 isolated from Northwestern Shanxi sour porridge as research object,the fermentation process of L.brevis M-10 for EPS production was optimized by single factor and response surface experiments based on the optimized carbon source and nitrogen source using MRS medium.The results showed that the fermentation temperature had an extremely significant effect on the EPS production of L.brevis M-10(P<0.01),and the inoculum had a significant effect(P<0.05).The optimal fermentation process was obtained as follows:fermentation temperature 36℃,time 45 h,initial pH 6.2 and inoculum 1.0%(V/V).Under these optimal conditions,the EPS yield was(2.54±0.13)g/L,which was 2.42 times higher than that before optimization.

关 键 词：晋西北酸粥 短乳杆菌 胞外多糖 发酵工艺 响应面优化 

分 类 号：TS264.2[轻工技术与工程—发酵工程]