复合酶解南美白对虾虾头制备ACE抑制肽的工艺优化  

Optimization of ACE Inhibitory Peptide Preparation from the Head of Penaeus albus by Complex Enzymatic Hydrolysis

作　　者：秦莹 马丹妮 陆节堃 朱虎 余炼[1] 刘小玲[1] QIN Ying;MA Danni;LU Jiekun;ZHU Hu;YU Lian;LIU Xiaoling(College of Light Industry and Food Engineering,Guangxi University,Nanning 530004,Guangxi,China)

机构地区：[1]广西大学轻工与食品工程学院,广西南宁530004

出　　处：《食品研究与开发》2024年第13期120-128,149,共10页Food Research and Development

基　　金：广西创新驱动发展专项资金项目(桂科AA20302019-7);北海市科技项目(Bhsfs010-3-1)。

摘　　要：以南美白对虾虾头为原料,利用复合酶解法制备血管紧张素转化酶(angiotensin-converting enzyme,ACE)抑制肽。在传统酶解法基础上,对酶解效率较高的蛋白酶进行筛选和复配,结合双酶复合酶解法水解虾头蛋白。以ACE抑制率和水解度为指标,通过单因素和响应面试验对制备工艺进行优化,确定复合蛋白酶组合方式及配比等工艺参数。得到虾头蛋白源ACE抑制肽的最佳酶解条件为料液比1∶7(g/mL)、酶添加量400 U/g、初始pH7.5、酶解温度50℃、复合酶(胰蛋白酶∶风味蛋白酶)配比2.29∶1(U/U)、酶解时间2.17 h,在此条件下虾头蛋白水解度达71.48%,ACE抑制率达83.57%,酶解效率与优化前的单酶水解相比提高35.05%。结果表明南美白对虾虾头经胰蛋白酶和风味蛋白酶酶解后具有较高的ACE抑制活性。Angiotensin⁃converting enzyme(ACE)inhibitory peptide was prepared from Penaeus albinus by complex enzymatic hydrolysis.On the basis of the traditional enzymatic hydrolysis method,the proteases with high enzymatic hydrolysis efficiency were screened and combined,and the shrimp head protein was hydro⁃lyzed by complex enzymatic hydrolysis of double enzymes.With ACE inhibition rate and hydrolysis degree as indexes,the preparation process was optimized by single factor and response surface tests,and the combina⁃tion,ratio,and other process parameters of complex protease were determined.The optimal enzymatic hydroly⁃sis conditions to obtain ACE inhibitory peptides from shrimp head protein were as follows:solid⁃liquid ratio of 1∶7(g/mL),enzyme dosage of 400 U/g,initial pH value of 7.5,enzymatic hydrolysis temperature at 50℃,complex enzyme ratio(mass ratio of trypsin to flavor protease)of 2.29∶1(U/U),and enzymatic hydrolysis time of 2.17 h.Under these conditions,the hydrolysis degree of shrimp head protein reached 71.48%,and the ACE inhibition rate reached 83.57%.The enzymatic hydrolysis efficiency was increased by 35.05%compared with the enzymatic hydrolysis of a single enzyme before optimization.The results showed that the head of Penaeus albinus had higher ACE inhibition activity after enzymatic hydrolysis by trypsin and flavor protease.

关 键 词：虾头 复合酶解 ACE抑制率 水解度 工艺优化 

分 类 号：TS254.9[轻工技术与工程—水产品加工及贮藏工程]