OPA柱前衍生高效液相色谱法同时测定黄豆酱中氨基酸和生物胺的含量  

Determination of Amino Acids and Biogenic Amines in Soybean Paste by Pre-Column Derivatization High Performance Liquid Chromatography

作　　者：续颖 罗旗峰 XU Ying;LUO Qifeng(Guangdong Meiweixian Flavoring Foods Co.,Ltd.,Zhongshan 528437,China)

机构地区：[1]广东美味鲜调味食品有限公司,广东中山528437

出　　处：《食品安全导刊》2024年第11期98-100,105,共4页China Food Safety Magazine

摘　　要：本文采用OPA衍生黄豆酱中的组氨酸、酪氨酸、组胺和酪胺,再通过高效液相色谱进行分离测定。结果表明,该方法的相对标准偏差在0.04%~1.16%,回收率在93.4%~101.2%,检出限为6~9 mg·kg^(-1)。该方法能够满足黄豆酱组氨酸、酪氨酸、组胺和酪胺的检测需求。In this article,histidine,tyrosine,histamine,and tyramine in soybean paste were derivatized using OPA,followed by separation and determination using high-performance liquid chromatography.The results showed that the RSD of the method was 0.04%~1.16%,the recovery was 93.4%~101.2%,and the detection limit was 6~9 mg·kg^(-1).The method can meet the requirements of detection of histidine,tyrosine,histamine and tyramine during soybean paste fermentation.

关 键 词：生物胺 氨基酸 黄豆酱 高效液相色谱 OPA衍生 

分 类 号：TS264.24[轻工技术与工程—发酵工程] O657.72[轻工技术与工程—食品科学与工程]