传统发酵酸鱼中乳酸菌的分离筛选及其表型分析  

Isolation,Screening and Phenotypic Analysis of Lactic Acid Bacteria from Traditional Fermented Suanyu

作　　者：孙海鑫 李志勇[2] 陈智慧 赵新强 亢春雨[1] 孙纪录[1] Sun Haixin;Li Zhiyong;Chen Zhihui;Zhao Xinqiang;Kang Chunyu;Sun Jilu(College of Food Science and Technology,Hebei Agricultural University,Baoding 071000,Hebei;Office of the State Assets Committee,Hebei Normal University,Shijiazhuang 050024;Department of Food Technology,Qujing Agricultural School of Yunnan,Qujing 655000,Yunnan)

机构地区：[1]河北农业大学食品科技学院,河北保定07100 [2]河北师范大学国有资产委员会办公室,石家庄050024 [3]云南省曲靖农业学校生物技术部,云南曲靖655000

出　　处：《中国食品学报》2024年第6期380-391,共12页Journal of Chinese Institute Of Food Science and Technology

基　　金：河北省现代农业产业技术体系淡水养殖创新团队建设项目(HBCT2024300207)。

摘　　要：为了从传统发酵酸鱼中筛选出具有良好发酵性能和益生性能的乳酸菌,采用高通量测序技术分析2个产区的16份酸鱼样品的细菌多样性。采用透明圈法筛选乳酸菌,通过生理生化试验和16S rDNA序列分析进行鉴定。对获得菌株的发酵性能(产酸速率、耐酸性、耐盐性、氨基酸脱羧酶活性)和益生性能(耐胆盐、疏水性、自聚集性、耐药性、抑菌性)进行系统分析。结果表明,乳杆菌属、四联球菌属和魏斯氏菌属是酸鱼中的优势菌属,相对丰度分别为40.73%,34.27%和6.42%。共筛选出24株乳酸菌,包括13株植物乳植杆菌,1株福菜乳杆菌,2株福菜伴生乳杆菌,3株消化伴生乳杆菌,3株屎肠球菌,1株粪肠球菌和1株戊糖片球菌。其中,福菜乳杆菌和福菜伴生乳杆菌C.futsaii为在酸鱼中首次分离到。所得乳酸菌菌株的表型差异显著,其中植物乳植杆菌S18、S21和福菜伴生乳杆菌S20具备较好的发酵性能,菌株S18和S21具有良好的益生性能。菌株S18、S20与S21有潜力作为酸鱼的发酵剂。In order to screen lactic acid bacteria with good fermentation and probiotic properties from traditionally fermented Suanyu.The diversity of bacteria in Suanyu from 2 production areas was examined through high throughput sequencing technology.Lactic acid bacteria were screened by transparent circle method,and identified by physiological and biochemical experiments and 16S rDNA sequence analysis.The fermentation properties(acid production rate,acid tolerance,salt tolerance,amino acid decarboxylase activities)and probiotic properties(bile salt tolerance,hydrophobicity,auto-aggregation,antibiotic sensitivity,antibacterial activity)of the obtained strains were systematically analyzed.Results showed that Lactobacillus,Tetragenococcus and Weissella were the dominant bacteria in Suanyu,and three relative abundances were 40.73%,34.27% and 6.42%,respectively.24 strains of lactic acid bacteria were screened,including 13 strains of Lactiplantibacillus plantarum,1 strain of L.futsaii,2 strains of Companilactobacillus futsaii,3 strains of Companilactobacillus alimentarius,3 strains of Enterococcus faecium,1 strain of Enterococcus faecalis and 1 strain of Pediococcus pentosaceus.Among them,L.futsaii and C.futsaii were isolated from Suanyu for the first time.Significant differences in phenotypic characteristics of 24 strains of lactic acid bacteria.Among them,L.plantarum S18,S21 and C.futsaii S20 have good fermentation properties,and strains S18 and S21 also have good probiotic properties.Strains S18,S20 and S21 have the potential to be used as starters of Suanyu.

关 键 词：酸鱼 乳酸菌 分离 鉴定 发酵性能 益生性能 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]