内蒙古阿拉善驼乳制品中乳酸菌分离鉴定及微生物多样性研究  

Isolation and Identification of Lactic Acid Bacteria and Microbial Diversity in Camel Dairy Products from Alxa Region,Inner Mongolia

作　　者：苏茜 孙洁 杜萍 赵洁[1] 于洁[1] 陈永福[1] Su Qian;Sun Jie;Du Ping;Zhao Jie;Yu Jie;Chen Yongfu(Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Key Laboratory of Dairy Processing,Ministry of Agriculture and Rural Affairs,Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering,Inner Mongolia Agricultural University,Hohhot 010018)

机构地区：[1]内蒙古农业大学乳品生物技术与工程教育部重点实验室、农业农村部奶制品加工重点实验室、内蒙古自治区乳品生物技术与工程重点实验室,呼和浩特010018

出　　处：《中国食品学报》2024年第6期392-401,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：内蒙古自治区科技计划项目(2020GG0101);内蒙古自治区高等学校创新团队发展计划项目(NMGIRT2220)。

摘　　要：内蒙古传统乳制品是乳酸菌的优质培养基,为保藏内蒙古阿拉善地区驼乳制品中的乳酸菌资源,并研究其中微生物多样性,本研究分别采用纯培养方法与宏基因组测序技术进行分析。宏基因组分析表明:阿拉善地区鲜驼乳微生物多样性明显高于酸驼乳,18份样品中共鉴定到157个种。鲜驼乳的优势菌种为乳酸乳球菌(13.22%)和桂林不动杆菌(10.65%);酸驼乳的优势菌种为瑞士乳杆菌(72.66%)、马乳酒样乳杆菌(8.32%)和乳酸乳球菌(4.28%)。纯培养进一步从鲜驼乳和酸驼乳中分离到121株乳酸菌,鉴定为4个属的14个种,其中鲜驼乳的优势菌株为乳酸乳球菌(62.00%),酸驼乳的优势菌株为瑞士乳杆菌(40.00%)、马乳酒样乳杆菌(19.00%),与宏基因组测序结果一致。宏基因组测序联合纯培养法可有效实现驼乳样品微生物多样性分析与乳酸菌资源挖掘,为驼乳的工业化生产与乳酸菌资源利用提供理论依据。Inner Mongolia traditional dairy products are high quality medium for lactic acid bacteria.In order to preserve lactic acid bacteria resources in camel dairy products in Alxa region of Inner Mongolia and study the microbial diversity,the pure culture method and metagomic sequencing technology were respectively used to evaluate and preserve.Metagenomic results showed that the microbial diversity of fresh camel milk was significantly higher than that of sour camel milk,and 157 species were identified from 18 samples.Lactococcus lactis(13.22%)and Acinetobacter guillouiae(10.65%)were the dominant species.The dominant species were Lactobacillus helveticus(72.66%),Lactobacillus kefiranofaciens(8.32%)and Lactococcus lactis(4.28%).A total of 121 strains of Lactobacillus were further isolated from the samples by pure culture and identified as 14 species of 4 genera,in which the dominant specie of fresh camel milk is Lactococcus lactis(62.00%).The dominant species were the Lactobacillus helveticus(40.00%)and the Lactobacillus kefiranofaciens(19.00%),which were consistent with the metapomic results.The combination of metagomic sequencing and pure culture method effectively realized the microbial diversity analysis and Lactobacillus resource mining of camel milk samples,providing a theoretical basis for the industrial production of camel milk and the utilization of lactobacillus resources.

关 键 词：驼乳 乳酸菌 微生物多样性 宏基因组 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]