3种多酚对腐败希瓦氏菌的抑菌效果和机理  

Antibacterial Effects and Mechanisms of Three Polyphenols against Shewanella putrefaciens

作　　者：王晓芸 张婷 黄剑 石柳 陈胜 郭晓嘉 汪兰[2] 吴文锦[2] 孙卫青[1] 熊光权[2] WANG Xiaoyun;ZHANG Ting;HUANG Jian;SHI Liu;CHEN Sheng;GUO Xiaojia;WANG Lan;WU Wenjin;SUN Weiqing;XIONG Guangquan(College of Life Science,Yangtze University,Jingzhou 434025,China;Key Laboratory of Cold Chain Logistics Technology for Agro-product,Ministry of Agriculture and Rural Affairs,Agro-product Processing Research Sub-center of Hubei Innovation Center of Agriculture Science and Technology,Institute of Agro-product Processing and Nuclear Agricultural Technology,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)

机构地区：[1]长江大学生命科学学院,湖北荆州434025 [2]湖北省农业科学院农产品加工与核农技术研究所,湖北省农业科技创新中心农产品加工研究分中心,农业农村部农产品冷链物流技术重点实验室,湖北武汉430064

出　　处：《食品科学》2024年第16期1-9,共9页Food Science

基　　金：国家现代农业产业技术体系建设专项(CARS-46);湖北省自然科学基金杰青项目(2022CFA095);湖北省青年拔尖人才培养计划项目:湖北省农业科技创新中心2020年重大科技研发专项(2020-620-000-002-03)。

摘　　要：为探讨葡萄籽提取物(grape seed extract,GSE)、莲房原花青素(lotus seed proanthocyanidins,LSPC)和莲藕多酚提取物(lotus root polyphenol extract,LRPE)3种植物多酚对腐败希瓦氏菌(Shewanella putrefaciens)的抑菌机理。通过最小抑菌质量浓度(minimal inhibitory concentration,MIC)、生长曲线探究3种多酚对腐败希瓦氏菌的抑菌效果,并通过扫描电子显微镜观察、相对电导率测定、碘化丙啶(propidium iodide,PI)染色观察及碱性磷酸酶(alkaline phosphatase,AKP)活力、胞外蛋白含量、核酸泄漏、Na^(+)K^(+)-ATP酶活性及膜蛋白荧光等测定,探讨3种多酚的抑菌机理。结果表明:GSE、LRPE、LSPC对腐败希瓦氏菌的MIC分别为31.25、62.25、125.00μg/mL;菌体经3种植物多酚处理后,膜蛋白位置发生改变,荧光强度降低,菌体形态发生改变,表面出现凹陷皱缩,且生长明显被抑制,胞外AKP活力、菌悬液中核酸、胞外蛋白含量、相对电导率及PI摄入量显著增加,Na^(+)K^(+)-ATP酶发生一定程度地失活,导致细胞死亡。This study was performed to investigate the inhibitory mechanisms of 3 plant polyphenols,grape seed extract(GSE),lotus seed proanthocyanidins(LSPC)and lotus root polyphenol extract(LRPE),against Shewanella putrefaciens.Their antibacterial effects were determined in terms of minimum inhibitory concentration(MIC)and the growth curve of S.putrefaciens.By scanning electron microscopy(SEM),relative conductivity,propyridine iodide(PI)staining,alkaline phosphatase(AKP)activity,extracellular protein content,nucleic acid leakage,Na^(+)K^(+)-ATPase activity and membrane protein fluorescence analysis,the antibacterial mechanism was explored.The results showed that the MICs of GSE,LRPE and LSPC were 31.25,62.25 and 125.00μg/mL,respectively.After S.putrefaciens was treated with the polyphenols,the position of membrane proteins was changed,the fluorescence intensity was reduced,the morphology was altered,the surface became wrinkled and sunken,and the growth was significantly inhibited.In addition,the activity of extracellular AKP,the contents of nucleic acid and extracellular protein in the bacterial suspension,relative conductivity and PI intake were significantly increased,and Na^(+)K^(+)-ATPase was inactivated to a certain extent,thereby leading to cell death.

关 键 词：腐败希瓦氏菌 葡萄籽提取物 莲房原花青素 莲藕多酚提取物 抑菌机理 

分 类 号：S983[农业科学—捕捞与储运]