河套麦胚多肽的制备工艺优化及其体外降血脂活性  

Preparation Optimization of Hetao Wheat Germ Polypeptide and Its in Vitro Hypolipidemic Activity

作　　者：李雨欣 胡芸利 刘聪 边瑞琴 包小兰[1] 王吉力特 LI Yuxin;HU Yunli;LIU Cong;BIAN Ruiqin;BAO Xiaolan;WANG Jilite(College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China;Department of Agriculture,Hetao College,Bayannur 015000,China)

机构地区：[1]内蒙古农业大学食品科学与工程学院,内蒙古呼和浩特010018 [2]河套学院农学系,内蒙古巴彦淖尔015000

出　　处：《食品工业科技》2024年第17期174-180,共7页Science and Technology of Food Industry

基　　金：“科技兴蒙”行动重点专项巴彦淖尔国家农业高新技术产业示范区重点项目(NMKJXM202111);河套学院科学技术研究项目(HYZX202165);内蒙古自治区自然科学基金资助项目(2021MS03028);巴彦淖尔市科技计划项目(K202105);河套特色农畜产品营养与高值化利用研究中心。

摘　　要：本文以河套麦胚蛋白为原料,采用碱性蛋白酶对其进行酶解,以水解度为指标,通过单因素及正交试验优化其工艺条件,并进行体外降血脂作用研究。结果表明,在底物浓度为6.0%,温度为50℃、pH为10.5及加酶量为12000 U/g的条件下,麦胚蛋白的水解度达到37.18%±0.25%;麦胚多肽5 mg/mL时胆固醇胶束溶解度抑制率为27.11%±0.36%,胰脂肪酶活性抑制率为33.54%±0.57%,胆固醇酯酶活性抑制率为38.13%±0.29%;超滤后相对分子质量小于1 kDa的多肽组分占54.30%±0.32%,说明小分子量的胚芽多肽可能具有较好的降血脂作用。上述结果为进一步研究河套胚芽多肽降血脂提供了理论依据。In this paper,alkali protease was used to enzymatically hydrolyze wheat germ proteins grown in the Hetao region.A univariate analysis and an orthogonal experiment were conducted to optimize the enzymatically hydrolyze process conditions of wheat germ proteins,with the degree of hydrolysis taken as the indicator.Moreover,the in vitro hypolipidemic effect of wheat germ polypeptides was investigated.The results showed that the degree of hydrolysis of wheat germ proteins was 37.18%±0.25%achieved by the following conditions,substrate concentration of 6.0%,temperature at 50℃,pH of 10.5 and protease amount of 12000 U/g.When the concentration of wheat germ polypeptides was 5 mg/mL,the rate of inhibiting cholesterol solubilisation into micelles was 27.11%±0.36%,the pancreatic lipase activity inhibition rate was 33.54%±0.57%,and cholesterol esterase activity inhibition rate was 38.13%±0.29%.Moreover,after ultrafiltration,the relative molecule weight of polypeptides less than 1 kDa accounted for 54.30%±0.32%of all polypeptides,indicating that low-molecular-weight wheat germ polypeptides might yield superior hypolipidemic effects.The work provides a theoretical foundation for further exploring the mechanism between Hetao wheat germ polypeptides and hypolipidemic effect.

关 键 词：麦胚蛋白 碱性蛋白酶 多肽 降血脂 超滤分离 

分 类 号：TS201.4[轻工技术与工程—食品科学]