一起副溶血性弧菌食物中毒事件中菌株全基因组测序与耐药性分析  

Whole genome sequencing and antibiotic resistance analysis of Vibrio parahaemolyticus isolated from a food poisoning incidents

作　　者：李兵兵[1] 张茹 刘靓[1] 李洁 陈大伟 李双姝 杨鹏飞[1] 刘纯成[1] 邢亚东[1] LI Bingbing;ZHANG Ru;LIU Liang;LI Jie;CHEN Dawei;LI Shuangshu;YANG Pengfei;LIU Chuncheng;XING Yadong(Huai’an Center for Disease Control and Prevention,Jiangsu Huai’an 223001,China;Xuyi Center for Disease Control and Prevention,Jiangsu Huai’an 211700,China)

机构地区：[1]淮安市疾病预防控制中心,江苏淮安223001 [2]盱眙县疾病预防控制中心,江苏淮安211700

出　　处：《中国食品卫生杂志》2024年第5期528-532,共5页Chinese Journal of Food Hygiene

基　　金：淮安市创新能力建设计划(HAP201906);江苏省预防医学科研课题(Y2018047)。

摘　　要：目的对2023年淮安市一起食物中毒事件分离的副溶血性弧菌进行全基因组测序和耐药性分析,为副溶血性弧菌引起的食源性疾病的防控和临床治疗提供参考。方法采用Illumina测序平台进行全基因组测序,通过全基因组单核苷酸多态性(wg-SNP)和多位点序列分型(MLST)分析进行溯源,在线数据库鉴定菌株携带的毒力和耐药基因,采用微量肉汤稀释法进行药敏实验。结果共分离到12株副溶血性弧菌,3株来自病例、9株来自留样食品。其中2株食物分离株与3株病例分离株wg-SNP差异数为0~2 bp,都属于ST3型,判断为同一克隆;其余7株食物分离株与病例分离株wg-SNP差异数为11341~37173 bp,判定为不同克隆。从病例和食品中分离的ST3型副溶血性弧菌都携带外毒素基因tdh和tlh,其余菌株仅携带tlh。本事件分离的副溶血性弧菌都携带blaCARB型耐药基因,且对头孢唑啉耐药率较高。结论通过全基因组测序分析,本起事件由携带tdh毒力基因的ST3型副溶血性弧菌污染食品引发,显示全基因组测序技术在中毒事件快速溯源中有较好的应用前景。该菌株对头孢唑啉耐药,可为临床治疗抗生素选用提供依据。Objective Whole genome sequencing and antibiotic resistance analysis were conducted for Vibrio parahaemolyticus strains isolated from a food poisoning incident in Huai’an City in 2023,so as to provide reference for the prevention and clinical treatment of foodborne diseases caused by Vibrio parahaemolyticus.Methods Whole genome sequencing was performed by Illumina sequencing platform,traceability was carried out by whole genome single nucleotide polymorphism(wg-SNPs)and multi-site sequence typing(MLST)analysis,the virulence and resistance genes carried by the strains were identified in the online database,and the antibiotic resistance was performed by microbroth dilution.Results Among the 12 strains of Vibrio parahaemolyticus,3 strains were isolated from patients and 9 strains were isolated from food samples.The difference of wg-SNPs of 3 strains from patients and 2 strains from food samples were 0~2 bp,both of which belonged to ST3 type and were judged to be the same clone.The difference of wg-SNPs between the remaining 7 strains from the food samples and the isolates from patients were 11341~37173 bp,which was judged to be different clones.Both the patients and the food-isolated Vibrio parahaemolyticus belonged to type ST3 carried the exotoxin genes tdh and tlh,while the remaining strains carried only tlh.All Vibrio parahaemolyticus strains isolated in this event carried durg resistance gene blaCARB and had a high rate of resistance to cefazoline.Conclusion Through whole genome sequencing analysis,the incident was caused by ST3 Vibrio parahaemolyticus carrying the tdh virulence gene and resistant to cefazoline,which showed promising application prospects of whole genome sequencing technology in rapid source tracing of poisoning events,and provided a basis for the selection of antibiotics in clinical treatment.

关 键 词：副溶血性弧菌 食物中毒 全基因组测序 耐药性 食源性致病菌 毒力基因 

分 类 号：R155[医药卫生—营养与食品卫生学]