液相色谱法检测原酒中7类天然甜味剂方法的研究  

Detection of Seven Natural Sweeteners in Crude Liquor by Liquid Chromatography

作　　者：朱艳 李先芝 熊晓通 毛琼丽 严玲 石豪 刘洋 胡杨 杨峰 ZHU Yan;LI Xianzhi;XIONG Xiaotong;MAO Qiongli;YAN Ling;SHI Hao;LIU Yang;HU Yang;YANG Feng(Jing Brand Co.Ltd.,Daye,Hubei 435100;Hubei Key Lab for Quality and Safety Control of Traditional Chinese Medicine and Healthcare Food,Daye,Hubei 435100,China)

机构地区：[1]劲牌有限公司,湖北大冶435100 [2]中药保健食品质量与安全湖北省重点实验室,湖北大冶435100

出　　处：《酿酒科技》2024年第9期124-134,共11页Liquor-Making Science & Technology

摘　　要：使用高效液相色谱仪串联蒸发光散射检测器,建立了一种可同时测定原酒中多种天然甜味剂(赤藓糖醇、核糖醇、山梨糖醇、甘露醇、异麦芽酮糖、麦芽糖醇、乳糖醇)含量的方法。采用乙腈-水(80∶20)等度洗脱,流速1.0 mL/min,柱温箱温度15℃,漂移管温度78.0℃,雾化气体流速2.1 L/min,进样量10μL。结果表明,原酒中赤藓糖醇、核糖醇、山梨糖醇、甘露醇、异麦芽酮糖、麦芽糖醇、乳糖醇在测定范围内呈良好的线性关系(R^(2)=0.995以上),平均回收率在94.36%~106.70%。该方法具有较高的精密度和重复性,可作为原酒中赤藓糖醇、核糖醇、山梨糖醇、甘露醇、异麦芽酮糖、麦芽糖醇、乳糖醇的含量测定方法。A method for simultaneous determination of seven natural sweeteners(erythritol,ribitol,sorbitol,mannitol,isomaltulose,maltitol,lactitol)in crude liquor was established by using high-performance liquid chromatography(HPLC)combined with evaporative light scattering detector(ELSD).Acetonitrile-water(80∶20)was used for isocratic elution,the flow rate was 1.0 mL/min,the column oven temperature was 15℃,the drift tube temperature was 78.0℃,the atomized gas flow rate was 2.1 L/min,and the injection amount was 10μL.The seven natural sweeteners showed good linear relationship(R^(2)≥0.995)within the detection range,with an average recovery of 94.36%to 106.70%.The method has high precision and repeatability,and can be used for the determination of erythritol,ribitol,sorbitol,mannitol,isomaltulose,maltitol and lactitol in crude liquor.

关 键 词：高效液相色谱仪 天然甜味剂 赤藓糖醇 核糖醇 山梨糖醇 甘露醇 异麦芽酮糖 麦芽糖醇 乳糖醇 

分 类 号：TS262.3[轻工技术与工程—发酵工程] TS261.7[轻工技术与工程—食品科学与工程]