含溶湿粕醇法制备大豆浓缩蛋白工艺优化及产品特性研究  

Optimization of the process and product characteristics of alcohol leacher soybean pro-tein concentrate prepared from solvent-containing meal

作　　者：杨迎 郭兴凤[1] 张明[2] 朱婷伟[1] 张相风 YANG Ying;GUO Xingfeng;ZHANG Ming;ZHU Tingwei;ZHANG Xiangfeng(College of Food Science and Engineering,Henan University of Technology,Zhengzhou 450001,China;Jining Machinery Design and Research Insitute,Jining 272023,China)

机构地区：[1]河南工业大学粮油食品学院,河南郑州450001 [2]济宁市机械设计研究院,山东济宁272023

出　　处：《河南工业大学学报（自然科学版）》2024年第5期25-32,96,共9页Journal of Henan University of Technology：Natural Science Edition

基　　金：国家重点研发计划(2022YFD2101403)。

摘　　要：为了优化醇法制备大豆浓缩蛋白的工艺,减少能源消耗,采用未经脱溶处理的含正己烷的脱脂大豆粕为原料制备浓缩蛋白,探究工艺条件对浓缩蛋白浸提效果以及原料对浓缩蛋白特性的影响。通过单因素及正交试验得到3个制备大豆浓缩蛋白的工艺条件:蛋白质含量优化组合条件制备蛋白的蛋白质含量66.32%、产品得率77.94%、蛋白质提取率93.98%;产品得率优化组合条件制备蛋白的蛋白质含量65.03%、产品得率80.17%、蛋白质提取率94.79%;蛋白质提取率优化组合条件制备蛋白的蛋白质含量65.33%、产品得率79.86%、蛋白质提取率94.86%。采用优化的工艺条件对比不同原料制备的大豆浓缩蛋白,发现与干粕制备的蛋白相比,含溶湿粕制备蛋白的蛋白质含量没有显著变化而溶解度提高,蛋白的表面疏水性、粒径、分子间疏水相互作用、氢键作用力降低,蛋白的溶解度与蛋白表面疏水性、疏水相互作用、氢键呈显著负相关。采用含溶湿粕醇法制备大豆浓缩蛋白的工艺更加简便,所制备的蛋白结构发生变化,溶解度更优。In order to optimize the extraction process of alcoholic soybean protein concentrate and reduce energy consumption,In this study,defatted soybean meal containing n-hexane without desolvation treatment was used as the raw materials for the preparation of alcoholic protein concentrate,and to investigate the influence of alcoholic processing conditions on the leaching effect of soybean protein concentrate and the influence of raw materials on the characteristics of alcoholic soybean protein concentrate.Three process conditions for the preparation of soybean protein concentrate were optimized by one-way and orthogonal experiments.Protein content of protein concentrate prepared under optimized combination of conditions of protein content,product yield and protein extraction rate are 66.32%,77.94% and 93.98%,respectively.Product yield optimized combination of conditions of protein concentrate prepared under optimized combination of conditions of protein content 65.03%,product yield 80.17%,protein extraction rate 94.79%;protein extraction rate optimized combination of conditions of protein concentrate prepared under optimized combination of conditions of protein content 65.33%,product yield 79.86%,protein extraction rate 94.86%.Protein content 65.33%,product yield 79.86%,protein extraction rate 94.86.The optimized process conditions were used to prepare soybean concentrate from different raw materials.It was found that compared with the protein prepared from dry meal,the protein content of the protein prepared from solvent-containing soluble wet meal did not change significantly,but the solubility increased,and the surface hydrophobicity,particle size,intermolecular hydrophobic interaction,and hydrogen bonding force of the protein decreased.The solubility of the protein was significantly negatively correlated with the surface hydrophobicity,hydrophobic interaction,and hydrogen bonding of the protein.The process of preparing soybean protein concentrate using the alcohol method with solvent-containing soluble wet meal is

关 键 词：含溶湿粕 大豆浓缩蛋白 工艺优化 溶解度 蛋白结构 

分 类 号：TS201.1[轻工技术与工程—食品科学]