Lacticaseibacillus paracasei K56 inhibits lipid accumulation in adipocytes by promoting lipolysis  

作　　者：Silu Wang Jinxing Li Wei-Hsien Liu Niya Li Huijing Liang Weilian Hung Qiuyue Jiang Ruyue Cheng Xi Shen Fang He 

机构地区：[1]Department of Nutrition and Food Hygiene,West China School of Public Health and West China Fourth Hospital,Sichuan University,Chengdu 610041,China [2]Inner Mongolia Yili Industrial Group Co.,Ltd.,Hohhot 010110,China

出　　处：《Food Science and Human Wellness》2024年第6期3511-3521,共11页食品科学与人类健康（英文）

基　　金：supported by the Yili Industrial Group Co., Ltd., and National Natural Science Foundation of China (81973042).

摘　　要：Probiotics crosstalk immunity to improve host glycolipid metabolism,which is a new strategy for obesity.This study aimed to explore the functions of Lacticaseibacillus paracasei K56(K56),in regulating the lipid metabolism of adipocytes and its immune regulation mechanisms.We co-cultured RAW264.7 macrophages with K56,and the K56-stimulated RAW264.7-conditioned medium(K56-CM)was collected and treated with 3T3-L1 pre-adipocytes.The expression of lipid metabolism-related markers of adipocytes,and the content of cytokines in CMs were detected.The results demonstrated that K56-CM promoted the expression of peroxisome proliferator-activated receptor-γ(PPAR-γ),CCAAT/enhancer binding proteinα(C/EBPα),and other adipogenesis-related markers,which resulted in the upregulation of lipolysis markers,such as hormone-sensitive lipase(HSL),adipose triglyceride lipase(ATGL).The activation of lipolysis enhanced the expression of fatty acidβ-oxidation-related and mitochondrial biogenesis-related markers and reduced lipid accumulation in adipocytes.The glycolipid metabolism pattern of K56 may be due to its immunomodulatory characteristics,which stimulated macrophages to secrete fewer TNF-α,thereby promoting the expression of lipolysis-related markers,and TNF-αsynergized with lipase to promote lipolysis.It has not been reported that L.paracasei modulated lipid metabolism via the lipolysis pathway,which suggested that K56 may regulate glycolipid metabolism of the host by maintaining immune homeostasis.

关 键 词：OBESITY Lacticaseibacillus MACROPHAGE ADIPOCYTE LIPOLYSIS 

分 类 号：TS201.3[轻工技术与工程—食品科学]